Positive Tested Species Reactivity: Bovine, Chicken, Drosophila, Equine, Human, Mouse, Primate, Rat
Depositors Notes: MC-480 recognizes mouse embryos, mouse embryonic cells (EC), mouse embryonic stem cells (ES) and mouse & human embryonic germ cells (EG). MC-480 does not bind to human EC, ES or iPS cells.
Predicted Species Reactivity:
Immunogen: Irradiated testis embryonal carcinoma F9 cell line
Alternate Gene Names:
Isotype: MIgM, kappa light chain
Alternate Antibody Name: anti-CD15, anti-Lewis X
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3-X63-Ag8
Epitope Location or Sequence: SSEA-1 is a carbohydrate epitope on glycolipids and glycoproteins involving fucosylated [GalBeta1-4(FucAlpha1-3)GlcNAcBeta1-R] type 2 blood group chains.
Additional Information: For experiments involving live cells and tissue, MC-480 supernatant is available without the antimicrobial ProClin. Contact us at 319-335-3826 or firstname.lastname@example.org for special ordering instructions.
Recommended Applications: FACS, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MC-480 (SSEA-1) was deposited to the DSHB by Solter, D. / Knowles, B.B (DSHB Hybridoma Product MC-480 (SSEA-1))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.