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N2 7A1 Armadillo
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Antigen: Armadillo segment polarity protein

Hybridoma Cells Available: Yes
Antigen Species: Drosophila Depositor: Wieschaus, E.
Antigen Sequence: Depositors Institution: Princeton University
Antigen Molecular Weight: 110 kDa Human Protein Atlas: Link
Predicted Species Reactivity:   Depositors Notes: This antigen is also known as armadillo drosophila protein. Armadillo is the mammalian homolog of beta-catenin.
Gene: arm Immunogen: Recombinant ATH-fusion protein
Alternate Gene Names: Immunogen Sequence: N-terminal region bp 2011-2175
Isotype: MIgG2a, kappa light chain Alternate Antibody Name:
Clonality: Monoclonal Alternate Antigen Name:
Epitope Mapped: Yes Myeloma Strain: SP2/0
Epitope Location or Sequence: Uniprot ID:
Host Species: mouse Entrez Gene ID:
Additional Characterization: Link Antibody Registry ID: AB_528089
Additional Information: FLYBASE:FBgn0000117  
Positive Tested Species Reactivity: Drosophila, Mouse Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N2 7A1 Armadillo was deposited to the DSHB by Wieschaus, E. (DSHB Hybridoma Product N2 7A1 Armadillo)

Storage and Handling Recommendations Usage Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored either at -20°C or -80°C. While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.  

Initial Publication

IHC References

IP References

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