AMV-3C2

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DSHB Data Sheet
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Product Name/ID: AMV-3C2
Available to For-Profits: Yes
Alternate Antibody Name: 3C2
Gene Name: gag-pol
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528098 
Uniprot ID: P03322 
RRID:  
Entrez Gene ID: P03322 
Clonality: Monoclonal
Immunogen: Avian Myeloblastosis Virus purified from infected chickens.
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: Yes
Antigen Name: avian myeloblastosis virus, p19^(gag)
Epitope Location or Sequence: N-terminal a.a.1-51 near a.a. 40.
Alternate Antigen Name:
Deposit Date: 5/5/1994
Antigen Molecular Weight: Predicted: 16.5kDa; Apparent: 19kDa
Depositor: Boettiger, D.
Antigen Sequence:
Depositor Institution: University of Pennsylvania
Antigen Species: Avian myeloblastosis virus (AMV)
Depositor Notes: This antibody identifies cells infected with all strains of the avian sarcoma/leukemia virus complex and can also be used to identify cells harboring Rous sarcoma or avian leukemia virus-derived vectors. Gag-poly protein is cleaved into protein fragments; p19 represents the first 155 amino acids of the Gag-poly protein.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity:  
Additional Information: Abdominal-b has two isoforms, M and R; isoform R lacks the amino terminal 223 aa portion of isoform M. Both M and R isoforms are recognized by mAb 1A2E9.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AMV-3C2 was deposited to the DSHB by Boettiger, D. (DSHB Hybridoma Product AMV-3C2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

43 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • Epitope Map References
  • All References
    • Initial Publication

    Epitope mapping of monoclonal antibodies to gag protein p19 of avian sarcoma and leukaemia viruses.
    Vogt VM
    The Journal of general virology 68 ( Pt 12). (1987 Dec): 3177-82.

    IF References

    Differential actions of VEGF-A isoforms on perichondrial angiogenesis during endochondral bone formation.
    Shukunami C
    Developmental biology 332.2 (2009 Aug 15): 196-211.

    Effects of follistatin overexpression on cell differentiation in the chick embryo retina.
    Adler R
    Developmental biology 298.1 (2006 Oct 1): 272-84.

    Endogenous expression of ASLV viral proteins in specific pathogen free chicken embryos: relevance for the developmental biology research field.
    Canto-Soler MV
    BMC developmental biology 10. (2010 Oct 18): 106.

    Non-cell-autonomous planar cell polarity propagation in the auditory sensory epithelium of vertebrates.
    Fekete DM
    Developmental biology 352.1 (2011 Apr 1): 27-39.

    β-Catenin inactivation is a pre-requisite for chick retina regeneration.
    Del Rio-Tsonis K
    PloS one 9.7 (2014): e101748.

    Shaping organs by a wingless-int/Notch/nonmuscle myosin module which orients feather bud elongation.
    Chuong CM
    Proceedings of the National Academy of Sciences of the United States of America 110.16 (2013 Apr 16): E1452-61.

    Sustained in vivo inhibition of protein domains using single-chain Fv recombinant antibodies and its application to dissect RGMa activity on axonal outgrowth.
    Monnier PP
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.4 (2009 Jan 28): 1126-31.

    Hoxa-5 acts in segmented somites to regulate cervical vertebral morphology.
    Mansfield JH
    Mechanisms of development 130.4-5 (2013 Apr-May): 226-40.

    Distinct susceptibility of developing neurons to death following Bax overexpression in the chicken embryo.
    Yaginuma H
    Cell death and differentiation 13.3 (2006 Mar): 435-45.

    The hedgehog pathway is a modulator of retina regeneration.
    Del Rio-Tsonis K
    Development (Cambridge, England) 131.18 (2004 Sep): 4607-21.

    FGF acts directly on the somitic tendon progenitors through the Ets transcription factors Pea3 and Erm to regulate scleraxis expression.
    Tabin CJ
    Development (Cambridge, England) 131.16 (2004 Aug): 3885-96.

    NeuroD induces the expression of visinin and calretinin by proliferating cells derived from toxin-damaged chicken retina.
    Reh TA
    Developmental dynamics : an official publication of the American Association of Anatomists 229.3 (2004 Mar): 555-63.

    Assigning the positional identity of spinal motor neurons: rostrocaudal patterning of Hox-c expression by FGFs, Gdf11, and retinoids.
    Jessell TM
    Neuron 32.6 (2001 Dec 20): 997-1012.

    A search for factors specifying tonotopy implicates DNER in hair-cell development in the chick's cochlea.
    Hudspeth AJ
    Developmental biology 354.2 (2011 Jun 15): 221-31.

    WB References

    Epitope mapping of monoclonal antibodies to gag protein p19 of avian sarcoma and leukaemia viruses.
    Vogt VM
    The Journal of general virology 68 ( Pt 12). (1987 Dec): 3177-82.

    Self-inactivating alpharetroviral vectors with a split-packaging design.
    Schambach A
    Journal of virology 84.13 (2010 Jul): 6626-35.

    IHC References

    Roles of FGFR3 during morphogenesis of Meckel's cartilage and mandibular bones.
    Mina M
    Developmental biology 316.2 (2008 Apr 15): 336-49.

    The apical ectodermal ridge (AER) can be re-induced by wounding, wnt-2b, and fgf-10 in the chicken limb bud.
    Wada N
    Developmental biology 342.2 (2010 Jun 15): 157-68.

    Prolonged effect of BAFF on chicken B cell development revealed by RCAS retroviral gene transfer in vivo.
    Kaspers B
    Molecular immunology 47.7-8 (2010 Apr): 1619-28.

    Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways.
    Renshaw PF
    PloS one 9.8 (2014): e104937.

    The chicken frizzle feather is due to an α-keratin (KRT75) mutation that causes a defective rachis.
    Chuong CM
    PLoS genetics 8.7 (2012): e1002748.

    Regulation of Tbx22 during facial and palatal development.
    Neubüser A
    Developmental dynamics : an official publication of the American Association of Anatomists 239.11 (2010 Nov): 2860-74.

    A BMP-mediated transcriptional cascade involving Cash1 and Tlx-3 specifies first-order relay sensory neurons in the developing hindbrain.
    Cairine Logan C
    Mechanisms of development 122.7-8 (2005 Jul): 900-13.

    Frzb modulates Wnt-9a-mediated beta-catenin signaling during avian atrioventricular cardiac cushion development.
    Klewer SE
    Developmental biology 278.1 (2005 Feb 1): 35-48.

    Defects in chicken neuroretina misexpressing the BMP antagonist Drm/Gremlin.
    Marx M
    Developmental biology 283.2 (2005 Jul 15): 335-44.

    Molecular evidence for an activator-inhibitor mechanism in development of embryonic feather branching.
    Prum RO
    Proceedings of the National Academy of Sciences of the United States of America 102.33 (2005 Aug 16): 11734-9.

    Morphogenesis of chicken liver: identification of localized growth zones and the role of beta-catenin/Wnt in size regulation.
    Chuong CM
    Developmental biology 266.1 (2004 Feb 1): 109-22.

    RCAS-RNAi: a loss-of-function method for the developing chick retina.
    Cepko CL
    BMC developmental biology 6. (2006 Jan 22): 2.

    FFPE References

    Genomic organization, transcriptomic analysis, and functional characterization of avian α- and β-keratins in diverse feather forms.
    Li WH
    Genome biology and evolution 6.9 (2014 Aug 24): 2258-73.

    The chicken frizzle feather is due to an α-keratin (KRT75) mutation that causes a defective rachis.
    Chuong CM
    PLoS genetics 8.7 (2012): e1002748.

    Regulation of Tbx22 during facial and palatal development.
    Neubüser A
    Developmental dynamics : an official publication of the American Association of Anatomists 239.11 (2010 Nov): 2860-74.

    Epitope Map References

    Epitope mapping of monoclonal antibodies to gag protein p19 of avian sarcoma and leukaemia viruses.
    Vogt VM
    The Journal of general virology 68 ( Pt 12). (1987 Dec): 3177-82.

    All References

    Roles of FGFR3 during morphogenesis of Meckel's cartilage and mandibular bones.
    Mina M
    Developmental biology 316.2 (2008 Apr 15): 336-49.

    The apical ectodermal ridge (AER) can be re-induced by wounding, wnt-2b, and fgf-10 in the chicken limb bud.
    Wada N
    Developmental biology 342.2 (2010 Jun 15): 157-68.

    Prolonged effect of BAFF on chicken B cell development revealed by RCAS retroviral gene transfer in vivo.
    Kaspers B
    Molecular immunology 47.7-8 (2010 Apr): 1619-28.

    Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways.
    Renshaw PF
    PloS one 9.8 (2014): e104937.

    The chicken frizzle feather is due to an α-keratin (KRT75) mutation that causes a defective rachis.
    Chuong CM
    PLoS genetics 8.7 (2012): e1002748.

    Regulation of Tbx22 during facial and palatal development.
    Neubüser A
    Developmental dynamics : an official publication of the American Association of Anatomists 239.11 (2010 Nov): 2860-74.

    A BMP-mediated transcriptional cascade involving Cash1 and Tlx-3 specifies first-order relay sensory neurons in the developing hindbrain.
    Cairine Logan C
    Mechanisms of development 122.7-8 (2005 Jul): 900-13.

    Frzb modulates Wnt-9a-mediated beta-catenin signaling during avian atrioventricular cardiac cushion development.
    Klewer SE
    Developmental biology 278.1 (2005 Feb 1): 35-48.

    Defects in chicken neuroretina misexpressing the BMP antagonist Drm/Gremlin.
    Marx M
    Developmental biology 283.2 (2005 Jul 15): 335-44.

    Molecular evidence for an activator-inhibitor mechanism in development of embryonic feather branching.
    Prum RO
    Proceedings of the National Academy of Sciences of the United States of America 102.33 (2005 Aug 16): 11734-9.

    Morphogenesis of chicken liver: identification of localized growth zones and the role of beta-catenin/Wnt in size regulation.
    Chuong CM
    Developmental biology 266.1 (2004 Feb 1): 109-22.

    RCAS-RNAi: a loss-of-function method for the developing chick retina.
    Cepko CL
    BMC developmental biology 6. (2006 Jan 22): 2.

    Differential actions of VEGF-A isoforms on perichondrial angiogenesis during endochondral bone formation.
    Shukunami C
    Developmental biology 332.2 (2009 Aug 15): 196-211.

    Effects of follistatin overexpression on cell differentiation in the chick embryo retina.
    Adler R
    Developmental biology 298.1 (2006 Oct 1): 272-84.

    Endogenous expression of ASLV viral proteins in specific pathogen free chicken embryos: relevance for the developmental biology research field.
    Canto-Soler MV
    BMC developmental biology 10. (2010 Oct 18): 106.

    Non-cell-autonomous planar cell polarity propagation in the auditory sensory epithelium of vertebrates.
    Fekete DM
    Developmental biology 352.1 (2011 Apr 1): 27-39.

    β-Catenin inactivation is a pre-requisite for chick retina regeneration.
    Del Rio-Tsonis K
    PloS one 9.7 (2014): e101748.

    Shaping organs by a wingless-int/Notch/nonmuscle myosin module which orients feather bud elongation.
    Chuong CM
    Proceedings of the National Academy of Sciences of the United States of America 110.16 (2013 Apr 16): E1452-61.

    Sustained in vivo inhibition of protein domains using single-chain Fv recombinant antibodies and its application to dissect RGMa activity on axonal outgrowth.
    Monnier PP
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.4 (2009 Jan 28): 1126-31.

    Hoxa-5 acts in segmented somites to regulate cervical vertebral morphology.
    Mansfield JH
    Mechanisms of development 130.4-5 (2013 Apr-May): 226-40.

    Distinct susceptibility of developing neurons to death following Bax overexpression in the chicken embryo.
    Yaginuma H
    Cell death and differentiation 13.3 (2006 Mar): 435-45.

    The hedgehog pathway is a modulator of retina regeneration.
    Del Rio-Tsonis K
    Development (Cambridge, England) 131.18 (2004 Sep): 4607-21.

    FGF acts directly on the somitic tendon progenitors through the Ets transcription factors Pea3 and Erm to regulate scleraxis expression.
    Tabin CJ
    Development (Cambridge, England) 131.16 (2004 Aug): 3885-96.

    NeuroD induces the expression of visinin and calretinin by proliferating cells derived from toxin-damaged chicken retina.
    Reh TA
    Developmental dynamics : an official publication of the American Association of Anatomists 229.3 (2004 Mar): 555-63.

    Assigning the positional identity of spinal motor neurons: rostrocaudal patterning of Hox-c expression by FGFs, Gdf11, and retinoids.
    Jessell TM
    Neuron 32.6 (2001 Dec 20): 997-1012.

    A search for factors specifying tonotopy implicates DNER in hair-cell development in the chick's cochlea.
    Hudspeth AJ
    Developmental biology 354.2 (2011 Jun 15): 221-31.

    Epitope mapping of monoclonal antibodies to gag protein p19 of avian sarcoma and leukaemia viruses.
    Vogt VM
    The Journal of general virology 68 ( Pt 12). (1987 Dec): 3177-82.

    Self-inactivating alpharetroviral vectors with a split-packaging design.
    Schambach A
    Journal of virology 84.13 (2010 Jul): 6626-35.

    Genomic organization, transcriptomic analysis, and functional characterization of avian α- and β-keratins in diverse feather forms.
    Li WH
    Genome biology and evolution 6.9 (2014 Aug 24): 2258-73.

    Cell-autonomous inhibition of alpha 7-containing nicotinic acetylcholine receptors prevents death of parasympathetic neurons during development.
    Nishi R
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.43 (2007 Oct 24): 11501-9.

    Knockdown of tissue nonspecific alkaline phosphatase impairs neural stem cell proliferation and differentiation.
    Zimmermann H
    Neuroscience letters 485.3 (2010 Nov 26): 208-11.

    GATA-3 regulates the transcriptional activity of tyrosine hydroxylase by interacting with CREB.
    Kim KS
    Journal of neurochemistry 98.3 (2006 Aug): 773-81.

    Twist1 activity thresholds define multiple functions in limb development.
    Laufer E
    Developmental biology 347.1 (2010 Nov 1): 133-46.

    Repulsive guidance molecule plays multiple roles in neuronal differentiation and axon guidance.
    Chédotal A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 26.22 (2006 May 31): 6082-8.

    Expression and function of Dlx genes in the osteoblast lineage.
    Lichtler AC
    Developmental biology 316.2 (2008 Apr 15): 458-70.

    Regulation of the noradrenaline neurotransmitter phenotype by the transcription factor AP-2beta.
    Kim KS
    The Journal of biological chemistry 283.24 (2008 Jun 13): 16860-7.

    Deletion of a Cys-His motif from the Alpharetrovirus nucleocapsid domain reveals late domain mutant-like budding defects.
    Linial ML
    Virology 347.1 (2006 Mar 30): 226-33.

    Somatic tetraploidy in specific chick retinal ganglion cells induced by nerve growth factor.
    Frade JM
    Proceedings of the National Academy of Sciences of the United States of America 107.1 (2010 Jan 5): 109-14.

    Alterations in heart looping induced by overexpression of the tight junction protein Claudin-1 are dependent on its C-terminal cytoplasmic tail.
    Ryan AK
    Mechanisms of development 123.3 (2006 Mar): 210-27.

    cDermo-1 misexpression induces dense dermis, feathers, and scales.
    Brand-Saberi B
    Developmental biology 277.1 (2005 Jan 1): 42-50.

    Integrin alpha4beta1 function is required for cell survival in developing retina.
    Clegg DO
    Developmental biology 276.2 (2004 Dec 15): 416-30.

    Fgf-dependent Etv4/5 activity is required for posterior restriction of Sonic Hedgehog and promoting outgrowth of the vertebrate limb.
    McMahon AP
    Developmental cell 16.4 (2009 Apr): 600-6.

    SKI-1 and Furin generate multiple RGMa fragments that regulate axonal growth.
    Monnier PP
    Developmental cell 22.2 (2012 Feb 14): 391-402.

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