Antigen: cAMP-specific phosphodieserase PDE4A4
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Bolger, G.B.
Isotype: MIgG2a, kappa light chain
Host Species: mouse
Depositors Institution: University of Alabama at Birmingham
Positive Tested Species Reactivity: Human
Antigen Molecular Weight: 97 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Alternate Gene Names: DPDE2
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Location or Sequence:
Uniprot ID: P27815
Immunogen Sequence: a.a. 51-70 SAERAERERQPHRPIERADA
Entrez Gene ID: 5141
Antibody Registry ID: AB_2617202
Additional Information: RRID:AB_2617202
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1-21.2 was deposited to the DSHB by Bolger, G.B. (DSHB Hybridoma Product 1-21.2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).