Product Form Descriptions

Supernatant (-s) is hybridoma-conditioned culture media predominantly composed of animal-derived component free (ADCF) mAb growth medium. Residual FBS from hybridoma culture initiation is present at a concentration of < 1.5-2.0%. No BSA is added for antibody protein stabilization.

Concentrate (-c) is produced by ultrafiltration through a 50kD exclusion filter. Concentrate is not the same as purified antibody, but does contain 10 times the immunoglobulin found in the supernatant. Therefore, 100 µl of concentrate contains approximately the same amount of monoclonal antibody as 1 ml of supernatant. Concentrate is recommended only for protocols requiring high antibody concentration in a small volume (i.e., immunoprecipitation, microinjection or antibody conjugation). For most routine immunostaining or immunoblotting protocols, we recommend supernatant as the first option. 

Purified monoclonal antibodies (-y) are available in a very limited number of cases, and may be mouse or rabbit mAbs. The antibody host varies, but most are rabbit or goat. Due to limited availability, the DSHB distributes purified antibodies in 10 µl aliquots, typically containing between 0.5-2 µg/µl mAb. We provide recommended conentrations for different applications for each antibody in the depositor's comments on each antibody page at the website.

Polyclonal antibody sera (-p) deposited with the DSHB are limited in number and represent a small percentage of the overall inventory. The antibody host varies, but most are rabbit or goat. Due to its limited availability, the DSHB distributes polyclonal sera in 10 µl aliquots. We provide a recommended dilution for each antibody in the depositor’s comments on each antibody page at the website. 

Datasheets are available for download at the website. Antibody concentration is provided on each tube label.

Frozen Cells (-f) are a 1mL aliquot containing approximately 5x10⁶ to 1x10⁷ cells. They are shipped on dry ice. When a customer receives them they are either thawed and put in culture immediately or placed in liquid nitrogen storage until they are needed.

Growing Cells (-g) are a live culture containing approximately 2x10⁷ to 4x10⁷ cells derived from a single frozen aliquot. DSHB production staff thaws the cells, ensures they are healthy and growing well, and then ships them in 65mL of media in a T25 flask. When the customer receives them they are immediately placed in an incubator for culture.

Frozen and growing hybridoma cells are shipped with full identification data and an outline of recommended protocols for culture and passage. Not all hybridoma cell lines are available.