10.1.1

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$40.00
SKU: 10.1.1
View product citations for antibody 10.1.1 on CiteAb

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Available: 40

DSHB Data Sheet

Catalog Fields

Antigen: Chloride channel calcium activated 3A1; CLCA1 protein
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Farr, A.G .
Isotype: Syrian Hamster IgG
Antigen Sequence:
Host Species: Syrian hamster
Depositors Institution: Univ. Wash. School of Medicine
Positive Tested Species Reactivity: Mouse
Depositors Notes: This antibody also cross reacts with mouse CLCA2 protein
Antigen Molecular Weight: 92kDa unreduced; 95 kDa reduced
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Clca3a1
Immunogen: Thymic stromal cells
Alternate Gene Names: Clca1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: X63-Ag8.653
Epitope Location or Sequence:
Uniprot ID: Q91Z28 
Immunogen Sequence:
Entrez Gene ID: 12722 
Additional Characterization:  
Antibody Registry ID:  
Additional Information: Frozen sections post-fixed with acetone or paraformaldehyde are recommended.
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
10.1.1 was deposited to the DSHB by Farr, A.G . (DSHB Hybridoma Product 10.1.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FACS References
  • All References
  • Initial Publication

    A novel cytokine-responsive cell surface glycoprotein defines a subset of medullary thymic epithelium in situ.
    Kim A
    Journal of immunology (Baltimore, Md. : 1950) 150.4 (1993 Feb 15): 1160-71.

    IF References

    A novel cytokine-responsive cell surface glycoprotein defines a subset of medullary thymic epithelium in situ.
    Kim A
    Journal of immunology (Baltimore, Md. : 1950) 150.4 (1993 Feb 15): 1160-71.

    B lymphocyte-specific c-Myc expression stimulates early and functional expansion of the vasculature and lymphatics during lymphomagenesis.
    Iritani BM
    The American journal of pathology 163.6 (2003 Dec): 2233-45.

    Lymphatic endothelial murine chloride channel calcium-activated 1 is a ligand for leukocyte LFA-1 and Mac-1.
    Ruddell A
    Journal of immunology (Baltimore, Md. : 1950) 185.10 (2010 Nov 15): 5769-77.

    WB References

    Lymphatic endothelial murine chloride channel calcium-activated 1 is a ligand for leukocyte LFA-1 and Mac-1.
    Ruddell A
    Journal of immunology (Baltimore, Md. : 1950) 185.10 (2010 Nov 15): 5769-77.

    IHC References
    IP References

    A novel cytokine-responsive cell surface glycoprotein defines a subset of medullary thymic epithelium in situ.
    Kim A
    Journal of immunology (Baltimore, Md. : 1950) 150.4 (1993 Feb 15): 1160-71.

    FACS References

    A novel cytokine-responsive cell surface glycoprotein defines a subset of medullary thymic epithelium in situ.
    Kim A
    Journal of immunology (Baltimore, Md. : 1950) 150.4 (1993 Feb 15): 1160-71.

    Lymph node-resident lymphatic endothelial cells mediate peripheral tolerance via Aire-independent direct antigen presentation.
    Engelhard VH
    The Journal of experimental medicine 207.4 (2010 Apr 12): 681-8.

    All References

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