10-19(1)-C11 SPI6
Catalog Fields
Clone ID/Product Name: 10-19(1)-C11 SPI6
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol: SERPINB9a
Ab Isotype: RIgG2a, kappa light chain
Gene Name:
Antibody Registry ID: AB_2936810
Uniprot ID: O08797
Entrez Gene ID: 20723
Clonality: Monoclonal
Immunogen: Recombinant protein expressed in Pichia Pastoris
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: Sp2/0
Epitope Mapped: No
Antigen Name: SERPINB9 / SPI6
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/26/2023
Antigen Molecular Weight: 42.2 kD
Depositor: Bird, P.I.
Antigen Sequence:
Depositor Institution: Monash University, Melbourne, AU
Antigen Species: Mouse
Depositor Notes: Mouse Serpinb9a is an intracellular inhibitor of the cytoxic protease granzyme B found in (but not limited to) CD8+ T cells, natural killer cells, mast cells and CD8+ dendritic cells.
Host Species: rat
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Mouse
Additional Information: This antibody does not western blot.Works well in intracellular FACs
Recommended Applications: ELISA, FACS, Immunofluorescence
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
10-19(1)-C11 SPI6 was deposited to the DSHB by Bird, P.I. (DSHB Hybridoma Product 10-19(1)-C11 SPI6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
1 Reference
Initial Publication
Serpinb9 is a marker of antigen cross-presenting dendritic cells.
Bird PI
Molecular immunology 82. (2017 Feb): 50-56.