10D9G11

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$40.00
SKU: 10D9G11-s
View product citations for antibody 10D9G11 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Germ cell nuclear antigen 1; GCNA1
Depositor: Enders, George
Antigen Species: Mouse
Depositors Institution: University of Kansas Medical School
Isotype: RIgM
Depositors Notes: Over 100 publications have used this antibody to recognize germ cells. The protein target has recently be identified as the Gcna gene product. See PMID: 27718356
Host Species: rat
Gene: Gcna
Positive Tested Species Reactivity: Mouse, Rat
Alternate Gene Names: Gcna1
Antigen Molecular Weight: 80-110 kDa depending upon modification
Clonality: Monoclonal
Immunogen: Pachytene spermatocytes
Epitope Location or Sequence: 8-amino-acid tandem GE(P/M/S)E(S/T)EAK repeat
Myeloma Strain: SP2/N
Additional Information: The epitope is sensitive to glutarldehyde and trypsin. RRID:AB_2629436
Entrez Gene ID: 107425 
Antibody Registry ID: AB_2629436 
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
10D9G11 was deposited to the DSHB by Enders, George (DSHB Hybridoma Product 10D9G11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

14 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References

    HP1gamma function is required for male germ cell survival and spermatogenesis.
    Singh PB
    Epigenetics & chromatin 3.1 (2010 Apr 27): 9.

    Germ cell differentiation and synaptonemal complex formation are disrupted in CPEB knockout mice.
    Richter JD
    Developmental cell 1.2 (2001 Aug): 201-13.

    Mammalian BTBD12 (SLX4) protects against genomic instability during mammalian spermatogenesis.
    Cohen PE
    PLoS genetics 7.6 (2011 Jun): e1002094.

    Developmentally regulated expression of a mouse germ cell nuclear antigen examined from embryonic day 11 to adult in male and female mice.
    May JJ 2nd
    Developmental biology 163.2 (1994 Jun): 331-40.

    Postnatal regulation of germ cells by activin: the establishment of the initial follicle pool.
    Woodruff TK
    Developmental biology 298.1 (2006 Oct 1): 132-48.

    Murine Inhibin α-Subunit Haploinsufficiency Causes Transient Abnormalities in Prepubertal Testis Development Followed by Adult Testicular Decline.
    Loveland KL
    Endocrinology 156.6 (2015 Jun): 2254-68.

    Huntingtin interacting protein 1 Is a clathrin coat binding protein required for differentiation of late spermatogenic progenitors.
    Ross TS
    Molecular and cellular biology 21.22 (2001 Nov): 7796-806.

    Prepubertal mouse testis growth and maturation and androgen production are acutely sensitive to di-n-butyl phthalate.
    Itman C
    Endocrinology 154.9 (2013 Sep): 3460-75.

    DNA methylation is a primary mechanism for silencing postmigratory primordial germ cell genes in both germ cell and somatic cell lineages.
    Resnick JL
    Development (Cambridge, England) 133.17 (2006 Sep): 3411-8.

    Mammalian BTBD12 (SLX4) protects against genomic instability during mammalian spermatogenesis.
    Cohen PE
    PLoS genetics 7.6 (2011 Jun): e1002094.

    FFPE References
    Epitope Map References
    All References

    HP1gamma function is required for male germ cell survival and spermatogenesis.
    Singh PB
    Epigenetics & chromatin 3.1 (2010 Apr 27): 9.

    Germ cell differentiation and synaptonemal complex formation are disrupted in CPEB knockout mice.
    Richter JD
    Developmental cell 1.2 (2001 Aug): 201-13.

    Mammalian BTBD12 (SLX4) protects against genomic instability during mammalian spermatogenesis.
    Cohen PE
    PLoS genetics 7.6 (2011 Jun): e1002094.

    Developmentally regulated expression of a mouse germ cell nuclear antigen examined from embryonic day 11 to adult in male and female mice.
    May JJ 2nd
    Developmental biology 163.2 (1994 Jun): 331-40.

    Postnatal regulation of germ cells by activin: the establishment of the initial follicle pool.
    Woodruff TK
    Developmental biology 298.1 (2006 Oct 1): 132-48.

    Murine Inhibin α-Subunit Haploinsufficiency Causes Transient Abnormalities in Prepubertal Testis Development Followed by Adult Testicular Decline.
    Loveland KL
    Endocrinology 156.6 (2015 Jun): 2254-68.

    Huntingtin interacting protein 1 Is a clathrin coat binding protein required for differentiation of late spermatogenic progenitors.
    Ross TS
    Molecular and cellular biology 21.22 (2001 Nov): 7796-806.

    Prepubertal mouse testis growth and maturation and androgen production are acutely sensitive to di-n-butyl phthalate.
    Itman C
    Endocrinology 154.9 (2013 Sep): 3460-75.

    DNA methylation is a primary mechanism for silencing postmigratory primordial germ cell genes in both germ cell and somatic cell lineages.
    Resnick JL
    Development (Cambridge, England) 133.17 (2006 Sep): 3411-8.

    Regulated CPEB phosphorylation during meiotic progression suggests a mechanism for temporal control of maternal mRNA translation.
    Richter JD
    Genes & development 17.12 (2003 Jun 15): 1457-62.

    Segmental message stabilization as a mechanism for differential expression from the Zymomonas mobilis gap operon.
    Ingram LO
    Journal of bacteriology 173.1 (1991 Jan): 245-54.

    Pathogenicity of different strains of vaccinia virus.
    Andleigh HS
    Indian journal of medical sciences 23.5 (1969 May): 247-51.

    A microcomputer based catheter laboratory system for analysis of coronary blood flow in man.
    Swanton H
    Journal of biomedical engineering 5.3 (1983 Jul): 248-52.

    A widely employed germ cell marker is an ancient disordered protein with reproductive functions in diverse eukaryotes.
    Page DC
    eLife 5. (2016 Oct 8): .

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