10E5

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$40.00
SKU: 10E5-s
View product citations for antibody 10E5 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: integrin alphaIIb
Hybridoma Cells Available:
Antigen Species: human
Depositor: Coller, B.S.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: Laboratory of Blood and Vascular Biology, Rockefeller University
Positive Tested Species Reactivity: Human, Primate
Depositors Notes: Inhibits ligand (fibrinogen-von Willebrand factor) binding to integrin alphaIIbbeta3 and platelet aggregation; does not react with platelets of patients with Glanzmann thrombasthenia. This antibody doesn't appear to work for western blotting.
Antigen Molecular Weight: Predicted: 103, 110, and 113 kDa; Apparent: 140 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: ITGA2B
Immunogen: Human platelets
Alternate Gene Names: CD41, GPIIb, GT, GTA, HPA3, CD41B, ITGAB
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: X63-Ag8.653
Epitope Location or Sequence: alphaIIb cap subdomain
Uniprot ID: P17301 
Immunogen Sequence: Full length protein
Entrez Gene ID: 3674 
Additional Characterization:
Antibody Registry ID: AB_2617389 
Additional Information: 10E5 recognizes the alphaIIb in its native conformation associated to beta3 integrin.
Recommended Applications: ELISA, FACS, Function Blocking, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
10E5 was deposited to the DSHB by Coller, B.S. (DSHB Hybridoma Product 10E5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

10 References

  • Initial Publication
  • IP References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IP References
    FB References
    FACS References
    Epitope Map References
    All References

    A murine monoclonal antibody that completely blocks the binding of fibrinogen to platelets produces a thrombasthenic-like state in normal platelets and binds to glycoproteins IIb and/or IIIa.
    Sullivan CA
    The Journal of clinical investigation 72.1 (1983 Jul): 325-38.

    Integrin beta3 regions controlling binding of murine mAb 7E3: implications for the mechanism of integrin alphaIIbbeta3 activation.
    Coller BS
    Proceedings of the National Academy of Sciences of the United States of America 101.36 (2004 Sep 7): 13114-20.

    Collagen-platelet interactions: evidence for a direct interaction of collagen with platelet GPIa/IIa and an indirect interaction with platelet GPIIb/IIIa mediated by adhesive proteins.
    Steinberg MH
    Blood 74.1 (1989 Jul): 182-92.

    Structure-guided design of a high-affinity platelet integrin αIIbβ3 receptor antagonist that disrupts Mg²⁺ binding to the MIDAS.
    Coller BS
    Science translational medicine 4.125 (2012 Mar 14): 125ra32.

    Platelet aggregation induced by a monoclonal antibody to the A1 domain of von Willebrand factor.
    Baruch D
    Blood 91.10 (1998 May 15): 3792-9.

    Activation of recombinant alphaIIbbeta3 expressed in Chinese hamster ovary cells exposes different binding sites for fibrinogen or von Willebrand factor: evidence using monoclonal antibodies to alphaIIbbeta3.
    Baruch D
    British journal of haematology 116.3 (2002 Mar): 636-44.

    αIIbβ3 variants defined by next-generation sequencing: predicting variants likely to cause Glanzmann thrombasthenia.
    Coller BS
    Proceedings of the National Academy of Sciences of the United States of America 112.15 (2015 Apr 14): E1898-907.

    Structural basis for allostery in integrins and binding to fibrinogen-mimetic therapeutics.
    Springer TA
    Nature 432.7013 (2004 Nov 4): 59-67.

    Monoclonal antibodies to alphaVbeta3 (7E3 and LM609) inhibit sickle red blood cell-endothelium interactions induced by platelet-activating factor.
    Coller BS
    Blood 95.2 (2000 Jan 15): 368-74.

    Probing conformational changes in the I-like domain and the cysteine-rich repeat of human beta 3 integrins following disulfide bond disruption by cysteine mutations: identification of cysteine 598 involved in alphaIIbbeta3 activation.
    Kieffer N
    The Journal of biological chemistry 276.42 (2001 Oct 19): 38628-35.

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