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SKU: 10E5-s

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Available: 37

DSHB Data Sheet

Catalog Fields

Product Name/ID: 10E5
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: ITGA2B
Ab Isotype: MIgG2a
Gene Symbol:
Antibody Registry ID: AB_2617389 
Uniprot ID: P17301 
Entrez Gene ID: 3674 
Clonality: Monoclonal
Immunogen: Human platelets
Immunogen Sequence: Full length protein
Myeloma Strain: X63-Ag8.653
Epitope Mapped: Yes
Antigen Name: integrin alpha IIb
Epitope Location or Sequence: alpha IIb cap subdomain
Alternate Antigen Name:
Deposit Date: 3/3/2012
Antigen Molecular Weight: Predicted: 103, 110, and 113 kDa; Apparent: 140 kDa
Depositor: Coller, B.S.
Antigen Sequence:
Depositor Institution: Rockefeller University, Laboratory of Blood and Vascular Biology
Antigen Species: human
Depositor Notes: Inhibits ligand (fibrinogen-von Willebrand factor) binding to integrin alphaIIbbeta3 and platelet aggregation; does not react with platelets of patients with Glanzmann thrombasthenia. This antibody doesn't appear to work for western blotting.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human, Primate
Additional Information: 10E5 recognizes the alphaIIb in its native conformation associated to beta3 integrin.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, FACS, Function Blocking, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
10E5 was deposited to the DSHB by Coller, B.S. (DSHB Hybridoma Product 10E5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

11 References

  • Initial Publication
  • IF References
  • IP References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    IP References
    FB References
    FACS References
    Epitope Map References
    All References

    Decellularized dermis extracellular matrix alloderm mechanically strengthens biological engineered tunica adventitia-based blood vessels.
    Lam MT
    Scientific reports 11.1 (2021 May 31): 11384.

    A murine monoclonal antibody that completely blocks the binding of fibrinogen to platelets produces a thrombasthenic-like state in normal platelets and binds to glycoproteins IIb and/or IIIa.
    Sullivan CA
    The Journal of clinical investigation 72.1 (1983 Jul): 325-38.

    Integrin beta3 regions controlling binding of murine mAb 7E3: implications for the mechanism of integrin alphaIIbbeta3 activation.
    Coller BS
    Proceedings of the National Academy of Sciences of the United States of America 101.36 (2004 Sep 7): 13114-20.

    Collagen-platelet interactions: evidence for a direct interaction of collagen with platelet GPIa/IIa and an indirect interaction with platelet GPIIb/IIIa mediated by adhesive proteins.
    Steinberg MH
    Blood 74.1 (1989 Jul): 182-92.

    Structure-guided design of a high-affinity platelet integrin αIIbβ3 receptor antagonist that disrupts Mg²⁺ binding to the MIDAS.
    Coller BS
    Science translational medicine 4.125 (2012 Mar 14): 125ra32.

    Platelet aggregation induced by a monoclonal antibody to the A1 domain of von Willebrand factor.
    Baruch D
    Blood 91.10 (1998 May 15): 3792-9.

    Activation of recombinant alphaIIbbeta3 expressed in Chinese hamster ovary cells exposes different binding sites for fibrinogen or von Willebrand factor: evidence using monoclonal antibodies to alphaIIbbeta3.
    Baruch D
    British journal of haematology 116.3 (2002 Mar): 636-44.

    αIIbβ3 variants defined by next-generation sequencing: predicting variants likely to cause Glanzmann thrombasthenia.
    Coller BS
    Proceedings of the National Academy of Sciences of the United States of America 112.15 (2015 Apr 14): E1898-907.

    Structural basis for allostery in integrins and binding to fibrinogen-mimetic therapeutics.
    Springer TA
    Nature 432.7013 (2004 Nov 4): 59-67.

    Monoclonal antibodies to alphaVbeta3 (7E3 and LM609) inhibit sickle red blood cell-endothelium interactions induced by platelet-activating factor.
    Coller BS
    Blood 95.2 (2000 Jan 15): 368-74.

    Probing conformational changes in the I-like domain and the cysteine-rich repeat of human beta 3 integrins following disulfide bond disruption by cysteine mutations: identification of cysteine 598 involved in alphaIIbbeta3 activation.
    Kieffer N
    The Journal of biological chemistry 276.42 (2001 Oct 19): 38628-35.

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