10E5
Catalog Fields
Product Name/ID: 10E5
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: ITGA2B
Ab Isotype: MIgG2a
Gene Symbol:
Antibody Registry ID: AB_2617389
Uniprot ID: P17301
Entrez Gene ID: 3674
Clonality: Monoclonal
Immunogen: Human platelets
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: X63-Ag8.653
Epitope Mapped: Yes
Antigen Name: integrin alpha IIb
Epitope Location or Sequence: alpha IIb cap subdomain
Alternate Antigen Name:
Deposit Date: 3/3/2012
Antigen Molecular Weight: Predicted: 103, 110, and 113 kDa; Apparent: 140 kDa
Depositor: Coller, B.S.
Antigen Sequence:
Depositor Institution: Rockefeller University, Laboratory of Blood and Vascular Biology
Antigen Species: human
Depositor Notes: Inhibits ligand (fibrinogen-von Willebrand factor) binding to integrin alphaIIbbeta3 and platelet aggregation; does not react with platelets of patients with Glanzmann thrombasthenia. This antibody doesn't appear to work for western blotting.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human, Primate
Additional Information: 10E5 recognizes the alphaIIb in its native conformation associated to beta3 integrin.
Recommended Applications: ELISA, FACS, Function Blocking, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
10E5 was deposited to the DSHB by Coller, B.S. (DSHB Hybridoma Product 10E5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
11 References
Initial Publication
A murine monoclonal antibody that completely blocks the binding of fibrinogen to platelets produces a thrombasthenic-like state in normal platelets and binds to glycoproteins IIb and/or IIIa.
Sullivan CA
The Journal of clinical investigation 72.1 (1983 Jul): 325-38.