10F6 (reovirus)

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SKU: 10F6 (reovirus)
View product citations for antibody 10F6 (reovirus) on CiteAb

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Available: 15

DSHB Data Sheet

Catalog Fields

Antigen: reovirus mu1C
Available to For-Profits: Yes
Antigen Species: Mammalian orthoreovirus strain Type 3 Dearing (T3D)
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG2a, kappa light chain
Depositor: Dermody, T.S.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Type 1 Lang (T1L), Type 3 Dearing (T3D)
Depositors Institution: University of Pittsburgh School of Medicine
Antigen Molecular Weight: 72 kDa
Depositors Notes: Inhibits sigma1 binding to sialoglycophorin on red blood cells.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: T3D intact virus
Gene: M2
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3 Ag8.6.5.3
Epitope Mapped: No
Uniprot ID: P11078 
Epitope Location or Sequence: Epitope resides within the delta fragment of mu lC (amino acids 43 to 581 of mu1), likely to be located in close structural proximity to sigma1
Entrez Gene ID: 8437017 
Immunogen Sequence: Full length sequence
Antibody Registry ID: AB_2617399 
Additional Characterization:  
Recommended Applications: ELISA, Function Blocking, Immunoprecipitation, Western Blot
Additional Information: RRID:AB_2617399
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
10F6 (reovirus) was deposited to the DSHB by Dermody, T.S. (DSHB Hybridoma Product 10F6 (reovirus))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

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