10F6 (reovirus)

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SKU: 10F6 (reovirus)
View product citations for antibody 10F6 (reovirus) on CiteAb

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Available: 36

DSHB Data Sheet

Catalog Fields

Antigen: reovirus mu1C
Hybridoma Cells Available: Yes
Antigen Species: Mammalian orthoreovirus strain Type 3 Dearing (T3D)
Depositor: Dermody, T.S.
Isotype: MIgG2a, kappa light chain
Depositors Institution: University of Pittsburgh School of Medicine
Host Species: mouse
Depositors Notes: Inhibits sigma1 binding to sialoglycophorin on red blood cells.
Positive Tested Species Reactivity: Type 1 Lang (T1L), Type 3 Dearing (T3D)
Gene: M2
Antigen Molecular Weight: 72 kDa
Clonality: Monoclonal
Immunogen: T3D intact virus
Epitope Mapped: No
Myeloma Strain: P3 Ag8.6.5.3
Epitope Location or Sequence: Epitope resides within the delta fragment of mu lC (amino acids 43 to 581 of mu1), likely to be located in close structural proximity to sigma1
Uniprot ID: P11078 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 8437017 
Additional Information: RRID:AB_2617399
Antibody Registry ID: AB_2617399 
Recommended Applications: ELISA, Function Blocking, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
10F6 (reovirus) was deposited to the DSHB by Dermody, T.S. (DSHB Hybridoma Product 10F6 (reovirus))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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