10G10 (reovirus)

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SKU: 10G10 (reovirus)
View product citations for antibody 10G10 (reovirus) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: reovirus T3D sigma 3
Hybridoma Cells Available: Yes
Antigen Species: Mammalian orthoreovirus strain Type 3 Dearing (T3D)
Depositor: Dermody, T.S.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Pittsburgh School of Medicine
Positive Tested Species Reactivity: Type 1 Lang (T1L), Type 3 Dearing (T3D)
Depositors Notes: Inhibits sigma1 binding to sialoglycophorin on red blood cells.
Antigen Molecular Weight: 41 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: S4
Immunogen: T3D intact virus
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3 Ag8.6.5.3
Epitope Location or Sequence: Epitope resides within sigma 3
Uniprot ID: P07939 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 8437031 
Additional Characterization:
Antibody Registry ID: AB_2617400 
Additional Information: RRID:AB_2617400
Recommended Applications: ELISA, Function Blocking, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
10G10 (reovirus) was deposited to the DSHB by Dermody, T.S. (DSHB Hybridoma Product 10G10 (reovirus))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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