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SKU: 12-120-94/6

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: 12-120-94/6
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_1553672 
Uniprot ID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: csA (contact site A glycoprotein) partially purified plasma membrane fraction followed by Triton X-114 extraction of 80kDa particulate fraction.
Immunogen Sequence:
Myeloma Strain: Designation of PA1
Epitope Mapped: No
Antigen Name: csA (contact site A glycoprotein)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 6/4/2008
Antigen Molecular Weight: 80 kDa, 53kDa and 66kDa bands in tunicamycin-treated cells
Depositor: Gerisch, G.
Antigen Sequence:
Depositor Institution: Max-Planck-Institut fuer Biochemie, Cell Dynamics
Antigen Species: Dictyostelium discoideum
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Dictyostelium
Additional Information: RRID:AB_1553672
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
12-120-94/6 was deposited to the DSHB by Gerisch, G. (DSHB Hybridoma Product 12-120-94/6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • WB References
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  • WB References
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