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SKU: 12-5B11-s
View product citations for antibody 12-5B11 on CiteAb

In Stock

Available: 40

DSHB Data Sheet

Catalog Fields

Antigen: Muscarinic antagonist propylbenzilylcholine mustard
Hybridoma Cells Available: No
Antigen Species:
Depositor: Nathanson, N.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: U of Wash School of Medicine - Seattle
Positive Tested Species Reactivity:  
Depositors Notes: These antibodies recognize muscarinic agonists such as pilocarpine, but do not bind nicotinic or cholingergic ligands.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Immunogen: PrBCM-BSA
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP/2
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence: PrBCM-protein conjugate
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2721934 
Additional Information: RRID:AB_2721934
Recommended Applications: ELISA, Function Blocking
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
12-5B11 was deposited to the DSHB by Nathanson, N.M. (DSHB Hybridoma Product 12-5B11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • ELISA References
  • FB References
  • All References
  • Initial Publication
    ELISA References
    FB References
    All References

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