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SKU: 12-5B11-s
View product citations for antibody 12-5B11 on CiteAb

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Available: 40

DSHB Data Sheet

Catalog Fields

Antigen: Muscarinic antagonist propylbenzilylcholine mustard
Hybridoma Cells Available: No
Isotype: MIgG1
Depositor: Nathanson, N.M.
Host Species: mouse
Depositors Institution: U of Wash School of Medicine - Seattle
Immunogen: PrBCM-BSA
Depositors Notes: These antibodies recognize muscarinic agonists such as pilocarpine, but do not bind nicotinic or cholingergic ligands.
Myeloma Strain: SP/2
Clonality: Monoclonal
Antibody Registry ID: AB_2721934 
Epitope Mapped: No
Recommended Applications: ELISA, Function Blocking
Immunogen Sequence: PrBCM-protein conjugate
Additional Information: RRID:AB_2721934
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
12-5B11 was deposited to the DSHB by Nathanson, N.M. (DSHB Hybridoma Product 12-5B11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • ELISA References
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  • Initial Publication
    ELISA References
    FB References
    All References

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