Antigen: csA (contact site A glycoprotein)
Hybridoma Cells Available: Yes
Antigen Species: Dictyostelium discoideum
Depositor: Gerisch, G.
Depositors Institution: Max-Planck-Institut fuer Biochemie, Cell Dynamics
Host Species: mouse
Positive Tested Species Reactivity: Dictyostelium
Additional Information: The origin of the antibody is Dictyostelium discoideum strain AX2 and the adjuvant of Immunization was Alu-gel S-Adjuvant immunized in 1987. RRID:AB_1553681
Antigen Molecular Weight: 68 kDa fragment in tunicamycin-treated cells
Immunogen: csA (contact site A glycoprotein); 68 kDa fragment of HG 220 The antibody has partially purified plasma membrane fraction followed by Triton X-114 extraction of 80kDa particulate fraction.
Myeloma Strain: Designation of PA1
Antibody Registry ID: AB_1553681
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
123-353-1 was deposited to the DSHB by Gerisch, G. (DSHB Hybridoma Product 123-353-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).