12C6c

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$40.00
SKU: 12C6c
View product citations for antibody 12C6c on CiteAb

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Available: 36

DSHB Data Sheet

Catalog Fields

Antigen: Flag Octapeptide DYKDDDDK
Hybridoma Cells Available: Yes
Antigen Species: N.A.
Depositor: Alfandari, D.
Isotype: MIgG2c
Antigen Sequence: DYKDDDDK
Host Species: mouse
Depositors Institution: U Mass -Amherst
Positive Tested Species Reactivity: All
Depositors Notes: Binds to same epitope as Sigma's Anti-FLAG ® M2 Antibody.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Recombinant protein
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence: DYKDDDDK
Uniprot ID:  
Immunogen Sequence: Six1-FLAG
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: RRID:AB_2890618 
Additional Information:
Recommended Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
12C6c was deposited to the DSHB by Alfandari, D. (DSHB Hybridoma Product 12C6c)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

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