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SKU: 12F10-5F11
View product citations for antibody 12F10-5F11 on CiteAb

In Stock

Available: 39

DSHB Data Sheet

Catalog Fields

Antigen: Megator
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Johansen, Kristen M.
Isotype: MIgG1
Antigen Sequence: a.a. 1433-1703
Host Species: mouse
Depositors Institution: Iowa State University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Megator is a nucleoporin protein that contributes to chromatin-binding.
Antigen Molecular Weight: 262 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Mtor
Immunogen: Recombinant peptide
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Sp2
Epitope Location or Sequence: a.a. 1433-1703
Uniprot ID: A0A0B4KFE9 
Immunogen Sequence: a.a. 1433-1703
Entrez Gene ID: 36267 
Additional Characterization:
Antibody Registry ID: AB_2721935 
Additional Information: RRID:AB_2721935
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
12F10-5F11 was deposited to the DSHB by Johansen, Kristen M. (DSHB Hybridoma Product 12F10-5F11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References
    IHC References
    IP References

    Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator.
    Maiato H
    The Journal of cell biology 184.5 (2009 Mar 9): 647-57.

    EAST interacts with Megator and localizes to the putative spindle matrix during mitosis in Drosophila.
    Johansen KM
    Journal of cellular biochemistry 95.6 (2005 Aug 15): 1284-91.

    All References

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