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$45.00 to $425.00
SKU: 12F10-5F11
View product citations for antibody 12F10-5F11 on CiteAb

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Available: 159

DSHB Data Sheet

Catalog Fields

Antigen: Megator
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Johansen, Kristen M.
Host Species: mouse
Antigen Sequence: a.a. 1433-1703
Positive Tested Species Reactivity: Drosophila
Depositors Institution: Iowa State University
Antigen Molecular Weight: 262 kDa
Depositors Notes: Megator is a nucleoporin protein that contributes to chromatin-binding.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Recombinant peptide
Gene: Mtor
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: Sp2
Epitope Mapped: Yes
Uniprot ID: A0A0B4KFE9 
Epitope Location or Sequence: a.a. 1433-1703
Entrez Gene ID: 36267 
Immunogen Sequence: a.a. 1433-1703
Antibody Registry ID: AB_2721935 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Additional Information: RRID:AB_2721935
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
12F10-5F11 was deposited to the DSHB by Johansen, Kristen M. (DSHB Hybridoma Product 12F10-5F11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

6 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References
    IHC References
    IP References

    Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator.
    Maiato H
    The Journal of cell biology 184.5 (2009 Mar 9): 647-57.

    EAST interacts with Megator and localizes to the putative spindle matrix during mitosis in Drosophila.
    Johansen KM
    Journal of cellular biochemistry 95.6 (2005 Aug 15): 1284-91.

    All References

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