12G9

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$40.00
SKU: 12G9
View product citations for antibody 12G9 on CiteAb

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Available: 5

DSHB Data Sheet

Catalog Fields

Antigen: filament antigen
Hybridoma Cells Available: Yes
Antigen Species: Tetrahymena thermophila
Depositor: Frankel, J./Nelsen, E.M./Jerka-Dziadosz, M.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Cell Biology M. Nencki Institute of Experimental Biology
Positive Tested Species Reactivity: Protozoa, Tetrahymena, Vertebrate
Depositors Notes:
Antigen Molecular Weight: 140 kDa cortical protein in T. thermophila
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: Tetrahymena thermophila (a ciliated protozoan)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Designation of NS-1
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_531773 
Additional Information: The origin of the antibody is deciliated plasma membrane & surface cytoskeleton of Tetrahymena thermophila, isolated by procedure of Nozawa & Thompson (J. Cell Biol. 49, 712-721). RRID:AB_531773
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
12G9 was deposited to the DSHB by Frankel, J./Nelsen, E.M./Jerka-Dziadosz, M. (DSHB Hybridoma Product 12G9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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