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SKU: 12H9-4A2
View product citations for antibody 12H9-4A2 on CiteAb

In Stock

Available: 15

DSHB Data Sheet

Catalog Fields

Antigen: Chromator
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Johansen, Kristen M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Iowa State University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antigen functions as a cytoskeletal regulator of chromatin.
Antigen Molecular Weight: 130 kDa apparent/ 101 kDa predicted
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Chro
Immunogen: Recombinant peptide
Alternate Gene Names: Chro-RB, Dmel_CG10712
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Sp2
Epitope Location or Sequence:
Uniprot ID: Q86BS3 
Immunogen Sequence: a.a. 1-260
Entrez Gene ID: 40508 
Additional Characterization:
Antibody Registry ID: AB_2721936 
Additional Information: FBgn0044324. RRID:AB_2721936
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
12H9-4A2 was deposited to the DSHB by Johansen, Kristen M. (DSHB Hybridoma Product 12H9-4A2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • All References
  • Initial Publication
    IF References
    WB References
    IP References
    All References

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