132-250-1

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$40.00
SKU: 132-250-1
View product citations for antibody 132-250-1 on CiteAb

Please allow 4 -6 weeks for production and delivery.

DSHB Data Sheet

Catalog Fields

Antigen: Peptide II B 80kD
Hybridoma Cells Available: Yes
Antigen Species: Dictyostelium discoideum
Depositor: Gerisch, G.
Isotype: MIgG2a
Depositors Institution: Max-Planck-Institut fuer Biochemie
Host Species: mouse
Clonality: Monoclonal
Positive Tested Species Reactivity: Dictyostelium
Epitope Mapped: No
Immunogen: Dictyostelium discoideum
Additional Information: The adjuvant of immunization was Alu-gel S-Adjuvant. RRID:AB_10570328
Myeloma Strain: Sp2/01 with designation of PA1
Antibody Registry ID: AB_10570328 
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
132-250-1 was deposited to the DSHB by Gerisch, G. (DSHB Hybridoma Product 132-250-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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