135-409-16
Catalog Fields
Antigen: Cap32/34
Hybridoma Cells Available: Yes
Antigen Species: Dictyostelium discoideum
Depositor: Gerisch, G.
Isotype: MIgG1
Depositors Institution: Max-Planck-Institut fuer Biochemie
Host Species: mouse
Clonality: Monoclonal
Positive Tested Species Reactivity: Dictyostelium
Additional Information: The adjuvan of immunization was AluGel in 1987. RRID:AB_1553425
Antigen Molecular Weight: 34 kDa
Immunogen: Cap32/34
Myeloma Strain: Designation of I: Ag8 II: PA1
Antibody Registry ID: AB_1553425
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
135-409-16 was deposited to the DSHB by Gerisch, G. (DSHB Hybridoma Product 135-409-16)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
2 References
Initial Publication
Ca2+-independent F-actin capping proteins. Cap 32/34, a capping protein from Dictyostelium discoideum, does not share sequence homologies with known actin-binding proteins.
Schleicher M
The Journal of biological chemistry 264.21 (1989 Jul 25): 12639-47.