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SKU: 13F4
View product citations for antibody 13F4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: muscle marker
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Le Douarin, N. / Rong, P.-M.
Isotype: MIgM
Depositors Institution: Institut d’Embryologie Cellulaire et Moleculaire du C.N.R.S. et du College de France
Host Species: mouse
Clonality: Monoclonal
Positive Tested Species Reactivity: Avian, Chicken, Quail
Additional Information: The immunization used 0.6 ml of homogenate in PBS 4 times with the intraperitoneal route. RRID:AB_531852
Antigen Molecular Weight: 54 kDa
Immunogen: homogenate of ganglion of Remak with surrounding dorsal mesentery
Myeloma Strain: Designation of Sp2/0
Antibody Registry ID: AB_531852 
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
13F4 was deposited to the DSHB by Le Douarin, N. / Rong, P.-M. (DSHB Hybridoma Product 13F4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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