141AA1
Catalog Fields
Clone ID/Product Name: 141AA1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: MIgM
Gene Name:
Antibody Registry ID: AB_2618048
Clonality: Monoclonal
Immunogen: Aggregatibacter actinomycetemcomitans, strain OMZ 250A (=Y4 or ATCC 43718), cell-free culture supernatant of 24 h culture in fluid medium
Clone:
Immunogen Sequence:
Myeloma Strain: Designation of F0
Epitope Mapped: No
Antigen Name: Aggregatibacter actinomycetemcomitans serotype b
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/1/2011
Antigen Molecular Weight:
Depositor: Gmuer, R.
Antigen Sequence:
Depositor Institution: University of Zuerich, Institute of Oral Biology, ZZMK
Antigen Species: A. actinomycetemcomitans
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: gram-negative bacteria
Additional Information: *Cell lines will be distributed only with the written consent of University of Zurich's tech transfer office, Ref. UZ-18/273. Contact the DSHB for details (dshb@uiowa.edu). The immunization of the antibody was in 1986 and used AlK(SO4) precipitate of approximately 100 ml culture supernatant. The route of immunization was intraperitoneal and the adjuvant was 50% incomplete Freund's adjuvant (1st immunization), none (2nd immunization). The origin of the antibody came from J. Slots, Univ. of Pennsylvania, Philadelphia, USA. RRID:AB_2618048
Recommended Applications: Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
141AA1 was deposited to the DSHB by Gmuer, R. (DSHB Hybridoma Product 141AA1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
7 References
Initial Publication
Monoclonal antibodies for the detection of 'periodontopathic' bacteria.
Guggenheim B
Archives of oral biology 35 Suppl. (1990): 145S-151S.