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SKU: 141AA1-s
View product citations for antibody 141AA1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Aggregatibacter actinomycetemcomitans serotype b
Depositor: Gmuer, R.
Antigen Species: A. actinomycetemcomitans
Depositors Institution: Institute of Oral Biology, ZZMK, University of Zuerich
Isotype: MIgM
Clonality: Monoclonal
Host Species: mouse
Additional Information: *Cell lines will be distributed only with the written consent of University of Zurich's tech transfer office, Ref. UZ-18/273. Contact the DSHB for details (dshb@uiowa.edu). The immunization of the antibody was in 1986 and used AlK(SO4) precipitate of approximately 100 ml culture supernatant. The route of immunization was intraperitoneal and the adjuvant was 50% incomplete Freund's adjuvant (1st immunization), none (2nd immunization). The origin of the antibody came from J. Slots, Univ. of Pennsylvania, Philadelphia, USA. RRID:AB_2618048
Positive Tested Species Reactivity: gram-negative bacteria
Immunogen: Aggregatibacter actinomycetemcomitans, strain OMZ 250A (=Y4 or ATCC 43718), cell-free culture supernatant of 24 h culture in fluid medium
Myeloma Strain: Designation of F0
Antibody Registry ID: AB_2618048 
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
141AA1 was deposited to the DSHB by Gmuer, R. (DSHB Hybridoma Product 141AA1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • All References
  • Initial Publication

    Monoclonal antibodies for the detection of 'periodontopathic' bacteria.
    Guggenheim B
    Archives of oral biology 35 Suppl. (1990): 145S-151S.

    All References

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