145AA1.1

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$40.00
SKU: 145AA1.1-s
View product citations for antibody 145AA1.1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Aggregatibacter actinomycetemcomitans serotype b
Hybridoma Cells Available: No
Antigen Species: A. actinomycetemcomitans
Depositor: Gmuer, R.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: Institute of Oral Biology, ZZMK, University of Zuerich
Positive Tested Species Reactivity: gram-negative bacteria
Depositors Notes:
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Aggregatibacter actinomycetemcomitans, strain OMZ 250A (= Y4, ATCC 43718), cell-free culture supernatant of 24 h culture in fluid medium
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Designation of F0
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2618049 
Additional Information: *Cell lines will be distributed only with the written consent of University of Zurich's tech transfer office, Ref. UZ-18/273. Contact the DSHB for details (dshb@uiowa.edu). The immunization of the antibody was in 1986 and used AlK(SO4) precipitate of approximately 100 ml culture supernatant. The route of immunization was intraperitoneal and the adjuvant was 50% incomplete Freund's adjuvant (1st immunization), none (2nd immunization). The origin of the antibody came from J. Slots, Univ. of Pennsylvania, Philadelphia, USA. RRID:AB_2618049
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
145AA1.1 was deposited to the DSHB by Gmuer, R. (DSHB Hybridoma Product 145AA1.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

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