(0) No Reviews yet
SKU: 145AA1.1-s
View product citations for antibody 145AA1.1 on CiteAb

In Stock

Available: 38

DSHB Data Sheet

Catalog Fields

Antigen: Aggregatibacter actinomycetemcomitans serotype b
Hybridoma Cells Available:
Antigen Species: A. actinomycetemcomitans
Depositor: Gmuer, R.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: Institute of Oral Biology, ZZMK, University of Zuerich
Positive Tested Species Reactivity: gram-negative bacteria
Depositors Notes:
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Immunogen: Aggregatibacter actinomycetemcomitans, strain OMZ 250A (= Y4, ATCC 43718), cell-free culture supernatant of 24 h culture in fluid medium
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Designation of F0
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_2618049 
Additional Information: *Cell lines will be distributed only with the written consent of University of Zurich's tech transfer office, Ref. UZ-18/273. Contact the DSHB for details (dshb@uiowa.edu). The immunization of the antibody was in 1986 and used AlK(SO4) precipitate of approximately 100 ml culture supernatant. The route of immunization was intraperitoneal and the adjuvant was 50% incomplete Freund's adjuvant (1st immunization), none (2nd immunization). The origin of the antibody came from J. Slots, Univ. of Pennsylvania, Philadelphia, USA. RRID:AB_2618049
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
145AA1.1 was deposited to the DSHB by Gmuer, R. (DSHB Hybridoma Product 145AA1.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • All References
  • Initial Publication

    Archives of oral biology
    Guggenheim B
    Archives of oral biology 35 Suppl. (1990): 145S-151S.

    All References

    Archives of oral biology
    Guggenheim B
    Archives of oral biology 35 Suppl. (1990): 145S-151S.

    Oral microbiology and immunology
    Guggenheim B
    Oral microbiology and immunology 8.2 (1993 Apr): 116-20.

    The Journal of laboratory and clinical medicine
    Hildebrand P
    The Journal of laboratory and clinical medicine 125.3 (1995 Mar): 378-83.

    Oral microbiology and immunology
    Baehni PC
    Oral microbiology and immunology 12.1 (1997 Feb): 1-10.

    Journal of clinical periodontology
    Frey J
    Journal of clinical periodontology 26.8 (1999 Aug): 505-10.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review