14a9

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$40.00
SKU: 14a9
View product citations for antibody 14a9 on CiteAb

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Available: 41

DSHB Data Sheet

Catalog Fields

Antigen: nuclear lamins II/III (Xenopus)
Hybridoma Cells Available: Yes
Antigen Species: Xenopus
Depositor: Klymkowsky, M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Molecular, Cellular & Developmental Biology, University of Colorado
Positive Tested Species Reactivity: Xenopus
Depositors Notes:
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: insoluble residues of Xenopus A6 (kidney epithelia), mainly intermediate filaments
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Designation of P3
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_531882 
Additional Information: During immunization a route of intraperitoneal was used and the adjuvant was RIBI. RRID:AB_531882
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
14a9 was deposited to the DSHB by Klymkowsky, M. (DSHB Hybridoma Product 14a9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    c-Jun N-terminal kinase phosphorylation of heterogeneous nuclear ribonucleoprotein K regulates vertebrate axon outgrowth via a posttranscriptional mechanism.
    Szaro BG
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.37 (2013 Sep 11): 14666-80.

    All References

    c-Jun N-terminal kinase phosphorylation of heterogeneous nuclear ribonucleoprotein K regulates vertebrate axon outgrowth via a posttranscriptional mechanism.
    Szaro BG
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.37 (2013 Sep 11): 14666-80.

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