14a9

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$40.00
SKU: 14a9
View product citations for antibody 14a9 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: nuclear lamins II/III (Xenopus)
Hybridoma Cells Available: Yes
Antigen Species: Xenopus
Depositor: Klymkowsky, M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Molecular, Cellular & Developmental Biology, University of Colorado
Positive Tested Species Reactivity: Xenopus
Depositors Notes:
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: insoluble residues of Xenopus A6 (kidney epithelia), mainly intermediate filaments
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Designation of P3
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_531882 
Additional Information: During immunization a route of intraperitoneal was used and the adjuvant was RIBI. RRID:AB_531882
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
14a9 was deposited to the DSHB by Klymkowsky, M. (DSHB Hybridoma Product 14a9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    c-Jun N-terminal kinase phosphorylation of heterogeneous nuclear ribonucleoprotein K regulates vertebrate axon outgrowth via a posttranscriptional mechanism.
    Szaro BG
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.37 (2013 Sep 11): 14666-80.

    All References

    c-Jun N-terminal kinase phosphorylation of heterogeneous nuclear ribonucleoprotein K regulates vertebrate axon outgrowth via a posttranscriptional mechanism.
    Szaro BG
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.37 (2013 Sep 11): 14666-80.

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