150AA1.1

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$40.00
SKU: 150AA1.1-s
View product citations for antibody 150AA1.1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Aggregatibacter actinomycetemcomitans serotype a
Hybridoma Cells Available:
Antigen Species: A. actinomycetemcomitans
Depositor: Gmuer, R.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: Institute of Oral Biology, ZZMK, University of Zuerich
Positive Tested Species Reactivity: gram-negative bacteria
Depositors Notes:
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: Aggregatibacter actinomycetemcomitans, strains OMZ 346A, intact bacteria, pasteurized
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Designation of F0
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_2618050 
Additional Information: *Cell lines will be distributed only with the written consent of University of Zurich's tech transfer office, Ref. UZ-18/273. Contact the DSHB for details (dshb@uiowa.edu). Immunization on 27 February 1986, and 21 March 1986 used approximately 10 to the 8th of bacteria through an intraperitoneal route. The origin of the antibody was through isolate from subgingival plaque. RRID:AB_2618050
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
150AA1.1 was deposited to the DSHB by Gmuer, R. (DSHB Hybridoma Product 150AA1.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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