159-183-10

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$40.00
SKU: 159-183-10
View product citations for antibody 159-183-10 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Proteasome subunit
Hybridoma Cells Available: Yes
Antigen Species: Dictyostelium discoideum
Depositor: Gerisch, G.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: Max-Planck-Institut fuer Biochemie, Cell Dynamics
Positive Tested Species Reactivity: Dictyostelium
Depositors Notes:
Antigen Molecular Weight: 34 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: Proteasomen subunit, Triton X-100 insoluble fraction.
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Designation of I: Ag 8 II:PAI
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_1553761 
Additional Information: The origin of the antibody comes from Dictyostelium discoideum strain HG1445. The immunization of the antibody used Alugel S with Freund’s complete adjuvant. RRID:AB_1553761
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
159-183-10 was deposited to the DSHB by Gerisch, G. (DSHB Hybridoma Product 159-183-10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Proteasomes from Dictyostelium discoideum: characterization of structure and function.
    Baumeister W
    Journal of structural biology 111.2 (1993 Sep-Oct): 135-47.

    All References

    Proteasomes from Dictyostelium discoideum: characterization of structure and function.
    Baumeister W
    Journal of structural biology 111.2 (1993 Sep-Oct): 135-47.

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