159-291-1

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$40.00
SKU: 159-291-1
View product citations for antibody 159-291-1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: actin binding protein 34 (ABP34)
Hybridoma Cells Available: Yes
Antigen Species: Dictyostelium discoideum
Depositor: Gerisch, G.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Max-Planck-Institut fuer Biochemie, Cell Dynamics
Positive Tested Species Reactivity: Dictyostelium
Depositors Notes:
Antigen Molecular Weight: 34 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: actin binding protein 34 (ABP34), aggregation competent cells
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Designation of PA1
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_10573113 
Additional Information: The immunization was adjuvant Alu-gel S-Adjuvant. The origin of the antibody was Dictyostelium discoideum strain AX2. RRID:AB_10573113
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
159-291-1 was deposited to the DSHB by Gerisch, G. (DSHB Hybridoma Product 159-291-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

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