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SKU: 15AE10
View product citations for antibody 15AE10 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: integrin beta-3 (CD61), human
Hybridoma Cells Available: Yes
Antigen Species: human
Depositor: Strom, D.
Isotype: MIgM
Depositors Institution: Marian University
Host Species: mouse
Depositors Notes: 15AE10 recognizes both the wild type integrin beta3 (HPA-1a) and the L33P variant (HPA-1b).
Positive Tested Species Reactivity: Human
Gene: ITGB3
Antigen Molecular Weight: Predicted; 87 kDa; Apparent: 90 kDa
Alternate Gene Names: GP3A, CD61
Immunogen: peptide CSDEALPLGSPRCDL (aa 26-40 of integrin beta3)
Clonality: Monoclonal
Uniprot ID: P05106 
Epitope Mapped: No
Entrez Gene ID: 3690 
Immunogen Sequence: peptide
Antibody Registry ID: AB_10567134 
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
15AE10 was deposited to the DSHB by Strom, D. (DSHB Hybridoma Product 15AE10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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