15AE10
Catalog Fields
Clone ID/Product Name: 15AE10
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: ITGB3
Ab Isotype: MIgM
Gene Name:
Antibody Registry ID: AB_10567134
Uniprot ID: P05106
Entrez Gene ID: 3690
Clonality: Monoclonal
Immunogen: peptide CSDEALPLGSPRCDL (aa 26-40 of integrin beta3)
Clone:
Immunogen Sequence: peptide
Myeloma Strain:
Epitope Mapped: No
Antigen Name: integrin beta-3 (CD61), human
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/19/2010
Antigen Molecular Weight: Predicted; 87 kDa; Apparent: 90 kDa
Depositor: Strom, D.
Antigen Sequence:
Depositor Institution: Marian University
Antigen Species: human
Depositor Notes: 15AE10 recognizes both the wild type integrin beta3 (HPA-1a) and the L33P variant (HPA-1b).
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information:
Recommended Applications: Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
15AE10 was deposited to the DSHB by Strom, D. (DSHB Hybridoma Product 15AE10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
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