Antigen: integrin beta-3 (CD61), human
Hybridoma Cells Available: Yes
Antigen Species: human
Depositor: Strom, D.
Depositors Institution: Marian University
Host Species: mouse
Depositors Notes: 15AE10 recognizes both the wild type integrin beta3 (HPA-1a) and the L33P variant (HPA-1b).
Positive Tested Species Reactivity: Human
Antigen Molecular Weight: Predicted; 87 kDa; Apparent: 90 kDa
Alternate Gene Names: GP3A, CD61
Immunogen: peptide CSDEALPLGSPRCDL (aa 26-40 of integrin beta3)
Uniprot ID: P05106
Epitope Mapped: No
Entrez Gene ID: 3690
Immunogen Sequence: peptide
Antibody Registry ID: AB_10567134
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
15AE10 was deposited to the DSHB by Strom, D. (DSHB Hybridoma Product 15AE10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).