Antigen: integrin beta-3 (CD61), human
Hybridoma Cells Available: Yes
Antigen Species: human
Depositor: Strom, D.
Host Species: mouse
Depositors Institution: Marian University
Positive Tested Species Reactivity: Human
Depositors Notes: 15AE10 recognizes both the wild type integrin beta3 (HPA-1a) and the L33P variant (HPA-1b).
Predicted Species Reactivity:
Immunogen: peptide CSDEALPLGSPRCDL (aa 26-40 of integrin beta3)
Alternate Gene Names: GP3A, CD61
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: No
Epitope Location or Sequence:
Uniprot ID: P05106
Immunogen Sequence: peptide
Entrez Gene ID: 3690
Antibody Registry ID: AB_10567134
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
15AE10 was deposited to the DSHB by Strom, D. (DSHB Hybridoma Product 15AE10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.