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SKU: 16-3C1
View product citations for antibody 16-3C1 on CiteAb

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Available: 6

DSHB Data Sheet

Catalog Fields

Antigen: transketolase
Hybridoma Cells Available: Yes
Antigen Species: Xenopus laevis
Depositor: Dreyer, C.
Isotype: MIgG1
Antigen Sequence: cDNA sequence-derived; AF270484.1 GI: 8515824 (Genbank)
Host Species: mouse
Depositors Institution: Max-Planck-Institut fuer Entwicklungsbiologie
Positive Tested Species Reactivity: Xenopus
Depositors Notes:
Antigen Molecular Weight: 67 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Immunogen: oocyte nuclear protein, total
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS-1 designation of P3-NS1-1-Ag4-1
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_531899 
Additional Information: The immunization used 70 micrograms of freund's incomplete adjuvant of antigen from 1979-1980. RRID:AB_531899
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
16-3C1 was deposited to the DSHB by Dreyer, C. (DSHB Hybridoma Product 16-3C1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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