16.5H2
Catalog Fields
Antigen: neuronal, motor, marker (SC-1)
Available to For-Profits: Yes
Antigen Species: chicken
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG2a, kappa light chain
Depositor: Jessell, T.M. / Dodd, J.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Avian
Depositors Institution: Columbia University
Antigen Molecular Weight: 100 kDa
Depositors Notes:
Immunogen: floor plate-homogenized tissue
Gene:
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: Designation of NS-1
Epitope Mapped: No
Antibody Registry ID: AB_531770
Recommended Applications: Western Blot
Additional Information: The immunization of 10 floor plates on 08/31/89; 09/15/89; 10/01/89; 10/30/89 used complete Freund’s adjuvant followed by incomplete Freund’s adjuvant. RRID:AB_531770
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
16.5H2 was deposited to the DSHB by Jessell, T.M. / Dodd, J. (DSHB Hybridoma Product 16.5H2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
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