169-477-5

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$40.00
SKU: 169-477-5
View product citations for antibody 169-477-5 on CiteAb

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Available: 43

DSHB Data Sheet

Catalog Fields

Antigen: talin
Hybridoma Cells Available: Yes
Antigen Species: Dictyostelium discoideum
Depositor: Gerisch, G.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: MPI for Biochemistry, Cell-Dynamics
Positive Tested Species Reactivity: Dictyostelium
Depositors Notes:
Antigen Molecular Weight: 205 kD
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: Talin, C-terminus, purified talin from actin-affinity column elute
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: PAIB3Ag8I
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_1553785 
Additional Information: The adjuvant used in immunization was alugel S. The origin of the antibody was Dictyostelium discoideum. RRID:AB_1553785
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
169-477-5 was deposited to the DSHB by Gerisch, G. (DSHB Hybridoma Product 169-477-5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    A talin homologue of Dictyostelium rapidly assembles at the leading edge of cells in response to chemoattractant.
    Müller-Taubenberger A
    The Journal of cell biology 129.1 (1995 Apr): 179-88.

    All References

    A talin homologue of Dictyostelium rapidly assembles at the leading edge of cells in response to chemoattractant.
    Müller-Taubenberger A
    The Journal of cell biology 129.1 (1995 Apr): 179-88.

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