Antigen: cardioactive peptide B
Hybridoma Cells Available: Yes
Antigen Species: Tritonia diomedea
Depositor: Kempf, S.C.
Antigen Sequence: MET:ASN:TYR:LEU:ALA:PHE:PRO:ARG:MET
Host Species: mouse
Depositors Institution: Auburn University
Positive Tested Species Reactivity: Invertebrate
Antigen Molecular Weight: SCPb 1.1414 kDa
Additional Information: Immunization was performed in 1984 and 100 µg of peptide-thyroglobulin conjugate was used. The injection was initiated subcutaneous injections followed by alternating subcutaneous and intraperitoneal injections and used complete Freund’s for the first immunization, incomplete Freund’s for the remainder. RRID:AB_2618098
Immunogen: small cardioactive peptide B, SCPB: MET:ASN:TYR:LEU:ALA:PHE:PRO:ARG:MET
Alternate Antibody Name: SCPb and SCP
Myeloma Strain: Designation of NS-1
Antibody Registry ID: AB_2618098
Recommended Applications: Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
17.1G6 was deposited to the DSHB by Kempf, S.C. (DSHB Hybridoma Product 17.1G6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).