Antigen: cardioactive peptide B
Hybridoma Cells Available: Yes
Antigen Species: Tritonia diomedea
Depositor: Kempf, S.C.
Antigen Sequence: MET:ASN:TYR:LEU:ALA:PHE:PRO:ARG:MET
Host Species: mouse
Depositors Institution: Auburn University
Positive Tested Species Reactivity: Invertebrate
Predicted Species Reactivity:
Immunogen: small cardioactive peptide B, SCPB: MET:ASN:TYR:LEU:ALA:PHE:PRO:ARG:MET
Alternate Gene Names:
Alternate Antibody Name: SCPb and SCP
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Designation of NS-1
Epitope Location or Sequence:
Antibody Registry ID: AB_2618098
Additional Information: Immunization was performed in 1984 and 100 µg of peptide-thyroglobulin conjugate was used. The injection was initiated subcutaneous injections followed by alternating subcutaneous and intraperitoneal injections and used complete Freund’s for the first immunization, incomplete Freund’s for the remainder. RRID:AB_2618098
Recommended Applications: Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
17.1G6 was deposited to the DSHB by Kempf, S.C. (DSHB Hybridoma Product 17.1G6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).