171-337

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$40.00
SKU: 171-337
View product citations for antibody 171-337 on CiteAb

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Available: 85

DSHB Data Sheet

Catalog Fields

Antigen: Proteasome subunit 5
Hybridoma Cells Available: Yes
Antigen Species: Dictyostelium discoideum
Depositor: Gerisch, G.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Cell-Dynamics, Max-Plank-Institut fuer Biochemie
Positive Tested Species Reactivity: Dictyostelium
Depositors Notes:
Antigen Molecular Weight: 29 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: proteasome subunit 5
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: I: Ag 8 II: PAI Ag 8: PAI=1:1
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_10584798 
Additional Information: Immunization used alugel as the adjuvant. RRID:AB_10584798
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
171-337 was deposited to the DSHB by Gerisch, G. (DSHB Hybridoma Product 171-337)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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