193E11E5B11

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$40.00
SKU: 193E11E5B11
View product citations for antibody 193E11E5B11 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: 30S Ribosomal protein S13 (E. coli)
Hybridoma Cells Available: Yes
Antigen Species: E. coli
Depositor: Kahan, L.
Isotype: MIgG1
Antigen Sequence: residues 84-117
Host Species: mouse
Depositors Institution: Univ of Wisc., Madison
Positive Tested Species Reactivity: E. coli
Depositors Notes: This antibody is also known as anti-Rps13/uS13.
Antigen Molecular Weight: 13 kDA
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: rpsM
Immunogen: Purified 30S ribosomal subunit 13
Alternate Gene Names: b3298, JW3260
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: a.a. 84-117
Uniprot ID: P0A7S9 
Immunogen Sequence: Full length protein
Entrez Gene ID: 947791 
Additional Characterization:
Antibody Registry ID: AB_2618084 
Additional Information: RRID:AB_2618084
Recommended Applications: ELISA, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
193E11E5B11 was deposited to the DSHB by Kahan, L. (DSHB Hybridoma Product 193E11E5B11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • WB References
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    Analytical biochemistry
    Kahan L
    Analytical biochemistry 196.1 (1991 Jul): 174-7.

    WB References

    Molecular cell
    Barna M
    Molecular cell 72.6 (2018 Dec 20): 1013-1020.e6.

    IP References

    Journal of protein chemistry
    Kahan L
    Journal of protein chemistry 11.3 (1992 Jun): 225-30.

    ELISA References

    Journal of immunological methods
    Kahan L
    Journal of immunological methods 118.2 (1989 Mar 31): 153-60.

    Epitope Map References

    Journal of protein chemistry
    Kahan L
    Journal of protein chemistry 8.6 (1989 Dec): 701-17.

    All References

    Molecular cell
    Barna M
    Molecular cell 72.6 (2018 Dec 20): 1013-1020.e6.

    Journal of protein chemistry
    Kahan L
    Journal of protein chemistry 11.3 (1992 Jun): 225-30.

    Journal of immunological methods
    Kahan L
    Journal of immunological methods 118.2 (1989 Mar 31): 153-60.

    Analytical biochemistry
    Kahan L
    Analytical biochemistry 196.1 (1991 Jul): 174-7.

    Journal of protein chemistry
    Kahan L
    Journal of protein chemistry 8.6 (1989 Dec): 701-17.

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