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SKU: 193E11E5B11

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 193E11E5B11
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: rpsM
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2618084 
Uniprot ID: P0A7S9 
Entrez Gene ID: 947791 
Clonality: Monoclonal
Immunogen: Purified 30S ribosomal subunit 13
Immunogen Sequence: Full length protein
Myeloma Strain: NS1
Epitope Mapped: Yes
Antigen Name: 30S Ribosomal protein S13 (E. coli)
Epitope Location or Sequence: a.a. 84-117
Alternate Antigen Name:
Deposit Date: 11/26/2011
Antigen Molecular Weight: 13 kDA
Depositor: Kahan, L.
Antigen Sequence: residues 84-117
Depositor Institution: University of Wisconsin-Madison, Dept. of Biomolecular Chemistry
Antigen Species: E. coli
Depositor Notes: This antibody is also known as anti-Rps13/uS13.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: E. coli
Additional Information: RRID:AB_2618084
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
193E11E5B11 was deposited to the DSHB by Kahan, L. (DSHB Hybridoma Product 193E11E5B11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

5 References

  • Initial Publication
  • WB References
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    WB References

    Decoding the Function of Expansion Segments in Ribosomes.
    Barna M
    Molecular cell 72.6 (2018 Dec 20): 1013-1020.e6.

    IP References
    ELISA References
    Epitope Map References

    Epitopes of Escherichia coli ribosomal protein S13.
    Kahan L
    Journal of protein chemistry 8.6 (1989 Dec): 701-17.

    All References

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