Antigen: ribosome, (E. coli)
Hybridoma Cells Available: Yes
Antigen Species: E. coli
Depositor: Kahan, L.
Antigen Sequence: residues 84-117
Host Species: mouse
Depositors Institution: Dept. of Biomolecular Chemistry, University of Wisconsin-Madison
Positive Tested Species Reactivity: E. coli
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: ribosomal proteins S13 + S14 + S19
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: NS-1
Epitope Location or Sequence:
Entrez Gene ID:
Antibody Registry ID: AB_2618085
Additional Information: RRID:AB_2618085
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
193F10C8C11 was deposited to the DSHB by Kahan, L. (DSHB Hybridoma Product 193F10C8C11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Journal of protein chemistry
Journal of protein chemistry 8.6 (1989 Dec): 701-17.