19H12TN-C
Catalog Fields
Clone ID/Product Name: 19H12TN-C
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: TNC
Ab Isotype: RIgG
Gene Name:
Antibody Registry ID: AB_2941796
Uniprot ID: P24821
Entrez Gene ID: 3371
Clonality: Monoclonal
Immunogen: Purified from embryonic fibroblast culture
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: X-Ag8-653
Epitope Mapped: Yes
Antigen Name: Tenascin-C, Fbn III domain A2
Epitope Location or Sequence: Fibronectin type III domain A2
Alternate Antigen Name:
Deposit Date: 1/24/2023
Antigen Molecular Weight: 240.8 kDa
Depositor: Faissner, Andreas
Antigen Sequence:
Depositor Institution: Ruhr University, Bochum, Germany
Antigen Species: Human
Depositor Notes: Does not cross react with mouse tenascin-C. This antibody recognizes the TNfnA2 domain in reduced and native proteins. Alternatively spliced TNfnA2 is expressed in tumor blood vessels. FFPE requires HIER using 10mM citrate buffer, pH 6.
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: mAb 19H12TN-C binds to perivascular single cells in tissues and to cell-cell interspaces and cell surfaces in vitro.
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
19H12TN-C was deposited to the DSHB by Faissner, Andreas (DSHB Hybridoma Product 19H12TN-C)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
1 Reference
Initial Publication
Tenascin-C is expressed by human glioma in vivo and shows a strong association with tumor blood vessels.
Faissner A
Cell and tissue research 354.2 (2013 Nov): 409-30.