Catalog Fields
Antigen: Skeletor
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgM
Depositor: Johansen, Kristen
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: Iowa State University
Antigen Molecular Weight: 81 kDa
Depositors Notes: This antigen is a component of the spindle matrix.
Immunogen: Recombinant peptide
Gene: Skeletor
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: Sp2
Epitope Mapped: Yes
Uniprot ID: Q9VGY6
Epitope Location or Sequence: specific to isoforms E and C
Entrez Gene ID: 3772559
Immunogen Sequence: a.a. 552-668
Antibody Registry ID: AB_2721937
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry
Additional Information: FBgn0262717 RRID:AB_2721937
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1A1-3C2 was deposited to the DSHB by Johansen, Kristen (DSHB Hybridoma Product 1A1-3C2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
1 Reference
Initial Publication
Skeletor, a novel chromosomal protein that redistributes during mitosis provides evidence for the formation of a spindle matrix.
Johansen KM
The Journal of cell biology 151.7 (2000 Dec 25): 1401-12.