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SKU: 1A1-3C2
View product citations for antibody 1A1-3C2 on CiteAb

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Available: 24

DSHB Data Sheet

Catalog Fields

Antigen: Skeletor
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Johansen, Kristen
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Iowa State University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antigen is a component of the spindle matrix.
Antigen Molecular Weight: 81 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Skeletor
Immunogen: Recombinant peptide
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Sp2
Epitope Location or Sequence: specific to isoforms E and C
Uniprot ID: Q9VGY6 
Immunogen Sequence: a.a. 552-668
Entrez Gene ID: 3772559 
Additional Characterization:
Antibody Registry ID: AB_2721937 
Additional Information: FBgn0262717 RRID:AB_2721937
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1A1-3C2 was deposited to the DSHB by Johansen, Kristen (DSHB Hybridoma Product 1A1-3C2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • IHC References
  • FB References
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