(0) No Reviews yet
SKU: 1A10-s
View product citations for antibody 1A10 on CiteAb

In Stock

Available: 148

DSHB Data Sheet

Catalog Fields

Antigen: oncomodulin (beta-parvalbumin)
Hybridoma Cells Available: No
Antigen Species: Rattus norvegicus
Depositor: Henzl, M.T.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Missouri
Positive Tested Species Reactivity: Gerbil, Guinea Pig, Human, Mouse, Rat
Depositors Notes: When immunostaining see J. Histochem. Cytochem. 46, 29-39 for a compatible immunostaining protocol. Epitope has not been mapped for thie antibody.
Antigen Molecular Weight: 12 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Immunogen: rat beta-parvalbumin (aka oncomodulin)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: PAI-O Mus musculus
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2618078 
Additional Information: RRID:AB_2618078
Recommended Applications: ELISA, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1A10 was deposited to the DSHB by Henzl, M.T. (DSHB Hybridoma Product 1A10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • All References
  • Initial Publication

    Oncomodulin is abundant in the organ of Corti.
    Thalmann R
    Hearing research 106.1-2 (1997 Apr): 105-11.

    All References

    Oncomodulin is abundant in the organ of Corti.
    Thalmann R
    Hearing research 106.1-2 (1997 Apr): 105-11.

    Population based models of cortical drug response: insights from anaesthesia.
    Liley DT
    Cognitive neurodynamics 2.4 (2008 Dec): 283-96.

    Oncomodulin is expressed exclusively by outer hair cells in the organ of Corti.
    Schulte BA
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 46.1 (1998 Jan): 29-40.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review