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SKU: 1A10-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 1A10
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2618078 
Uniprot ID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: rat beta-parvalbumin (aka oncomodulin)
Immunogen Sequence:
Myeloma Strain: PAI-O Mus musculus
Epitope Mapped: No
Antigen Name: oncomodulin (beta-parvalbumin)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 8/16/2012
Antigen Molecular Weight: 12 kDa
Depositor: Henzl, M.T.
Antigen Sequence:
Depositor Institution: University of Missouri
Antigen Species: Rattus norvegicus
Depositor Notes: When immunostaining see J. Histochem. Cytochem. 46, 29-39 for a compatible immunostaining protocol. Epitope has not been mapped for thie antibody.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Gerbil, Guinea Pig, Human, Mouse, Rat
Additional Information: RRID:AB_2618078
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1A10 was deposited to the DSHB by Henzl, M.T. (DSHB Hybridoma Product 1A10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

3 References

  • Initial Publication
  • All References
  • Initial Publication

    Oncomodulin is abundant in the organ of Corti.
    Thalmann R
    Hearing research 106.1-2 (1997 Apr): 105-11.

    All References

    Oncomodulin is abundant in the organ of Corti.
    Thalmann R
    Hearing research 106.1-2 (1997 Apr): 105-11.

    Population based models of cortical drug response: insights from anaesthesia.
    Liley DT
    Cognitive neurodynamics 2.4 (2008 Dec): 283-96.

    Oncomodulin is expressed exclusively by outer hair cells in the organ of Corti.
    Schulte BA
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 46.1 (1998 Jan): 29-40.

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