1A3G4

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$40.00
SKU: 1A3G4
View product citations for antibody 1A3G4 on CiteAb

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Available: 170

DSHB Data Sheet

Catalog Fields

Antigen: Frizzled-2, N-terminus
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Mayor, Satyajit
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: National Centre for Biological Sciences, Bangalore, India
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody recognizes the extracellular domain of DFz2, a receptor for Wnt proteins.
Antigen Molecular Weight: 75.4 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: fz2
Immunogen: Synthetic protein domain
Alternate Gene Names: Dmel_CG9739
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP/20
Epitope Location or Sequence:
Uniprot ID: Q9VVX3 
Immunogen Sequence: a.a. 22-114
Entrez Gene ID: 40090 
Additional Characterization:  
Antibody Registry ID: AB_2721938 
Additional Information: This antibody has also been used in an endocytosis assay using Drosophila wing discs. RRID:AB_2721938
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1A3G4 was deposited to the DSHB by Mayor, Satyajit (DSHB Hybridoma Product 1A3G4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    Endocytosis of Wingless via a dynamin-independent pathway is necessary for signaling in <i>Drosophila</i> wing discs.
    Mayor S
    Proceedings of the National Academy of Sciences of the United States of America 113.45 (2016 Nov 8): E6993-E7002.

    IF References

    Endocytosis of Wingless via a dynamin-independent pathway is necessary for signaling in <i>Drosophila</i> wing discs.
    Mayor S
    Proceedings of the National Academy of Sciences of the United States of America 113.45 (2016 Nov 8): E6993-E7002.

    WB References

    Endocytosis of Wingless via a dynamin-independent pathway is necessary for signaling in <i>Drosophila</i> wing discs.
    Mayor S
    Proceedings of the National Academy of Sciences of the United States of America 113.45 (2016 Nov 8): E6993-E7002.

    All References

    Endocytosis of Wingless via a dynamin-independent pathway is necessary for signaling in <i>Drosophila</i> wing discs.
    Mayor S
    Proceedings of the National Academy of Sciences of the United States of America 113.45 (2016 Nov 8): E6993-E7002.

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