Antigen: Frizzled-2, N-terminus
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Mayor, Satyajit
Isotype: MIgG2a, kappa light chain
Host Species: mouse
Depositors Institution: National Centre for Biological Sciences, Bangalore, India
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody recognizes the extracellular domain of DFz2, a receptor for Wnt proteins.
Antigen Molecular Weight: 75.4 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Synthetic protein domain
Alternate Gene Names: Dmel_CG9739
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: SP/20
Epitope Location or Sequence:
Uniprot ID: Q9VVX3
Immunogen Sequence: a.a. 22-114
Entrez Gene ID: 40090
Antibody Registry ID: AB_2721938
Additional Information: This antibody has also been used in an endocytosis assay using Drosophila wing discs. RRID:AB_2721938
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1A3G4 was deposited to the DSHB by Mayor, Satyajit (DSHB Hybridoma Product 1A3G4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).