1A4 CD141
Catalog Fields
Clone ID/Product Name: 1A4 CD141
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol: THBD
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_2936806
Uniprot ID: P07204
Entrez Gene ID: 7056
Clonality: Monoclonal
Immunogen: Purified human placental thrombomodulin
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: Sp2/0
Epitope Mapped: No
Antigen Name: Thrombomodulin (CD141)
Epitope Location or Sequence: a.a. 207-237
Alternate Antigen Name:
Deposit Date: 4/26/2023
Antigen Molecular Weight: 60.3 kDa
Depositor: Bird, P.I.
Antigen Sequence:
Depositor Institution: Monash University, Melbourne, AU
Antigen Species: Human
Depositor Notes: Thrombomodulin regulates blood coagulation and hemostasis.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human
Additional Information:
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1A4 CD141 was deposited to the DSHB by Bird, P.I. (DSHB Hybridoma Product 1A4 CD141)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
2 References
Initial Publication
Internalization of the anticoagulant thrombomodulin is constitutive and does not require a signal in the cytoplasmic domain.
Bird P
Immunology and cell biology 72.6 (1994 Dec): 480-8.