1A4 CD141

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$50.00
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SKU: 1A4 CD141-s

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: 1A4 CD141
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol: THBD
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_2936806 
Uniprot ID: P07204 
RRID:  
Entrez Gene ID: 7056 
Clonality: Monoclonal
Immunogen: Purified human placental thrombomodulin
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: Sp2/0
Epitope Mapped: No
Antigen Name: Thrombomodulin (CD141)
Epitope Location or Sequence: a.a. 207-237
Alternate Antigen Name:
Deposit Date: 4/26/2023
Antigen Molecular Weight: 60.3 kDa
Depositor: Bird, P.I.
Antigen Sequence:
Depositor Institution: Monash University, Melbourne, AU
Antigen Species: Human
Depositor Notes: Thrombomodulin regulates blood coagulation and hemostasis.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1A4 CD141 was deposited to the DSHB by Bird, P.I. (DSHB Hybridoma Product 1A4 CD141)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

2 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References

    Immunohistochemical localization of thrombomodulin in normal human skin and skin tumours.
    Hayman JA
    The Journal of pathology 175.4 (1995 Apr): 421-32.

    ELISA References
    Epitope Map References
    All References

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