1A8

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$40.00
SKU: 1A8
View product citations for antibody 1A8 on CiteAb

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Available: 34

DSHB Data Sheet

Catalog Fields

Antigen: Us9 (pseudorabies virus)
Hybridoma Cells Available: Yes
Antigen Species: pseudorabies virus
Depositor: Enquist, L. / Lyman, M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Dept. of Molecular Biology, Princeton University
Positive Tested Species Reactivity: Virus
Depositors Notes: The antibody is a type II membane protein which is essential for axonal sorting of PRV structural components.
Antigen Molecular Weight: 17-20 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: glutathione S-transferase-Us9 fusion protein
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_1553789 
Additional Information: The origin of the immunogen is expressed in Escheriachia coli DH5 alpha cells and isolated as inclusion bodies. Works with pseudorabies virus RRID:AB_1553789
Recommended Applications: Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1A8 was deposited to the DSHB by Enquist, L. / Lyman, M. (DSHB Hybridoma Product 1A8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    A self-recombining bacterial artificial chromosome and its application for analysis of herpesvirus pathogenesis.
    Enquist LW
    Proceedings of the National Academy of Sciences of the United States of America 97.9 (2000 Apr 25): 4873-8.

    All References

    A self-recombining bacterial artificial chromosome and its application for analysis of herpesvirus pathogenesis.
    Enquist LW
    Proceedings of the National Academy of Sciences of the United States of America 97.9 (2000 Apr 25): 4873-8.

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