1B2 JW

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$40.00
SKU: 1B2 JW
View product citations for antibody 1B2 JW on CiteAb

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Available: 5

DSHB Data Sheet

Catalog Fields

Antigen: hexokinase (Type I isozyme)
Hybridoma Cells Available: Yes
Antigen Species: rat
Depositor: Wilson, J.E.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: 301 Biochemistry, Dept. of Biochemistry & Molecular Biology, Michigan State University
Positive Tested Species Reactivity: Rat
Depositors Notes: The antibody is reactive with the native enzyme (ELISA) but not the denatured form of the enzyme and thus is not useful for immunoblots. Binding of the antibody does not affect the kinetic properties of the rat Type I isozyme [Smith and Wilson (1992) Arch. Biochem. Biophys. 292: 165-178]. The epitope recognized is a ÒconformationalÓ (or ÒdiscontinuousÓ) epitope, and immunoreactivity is affected by conformational changes in the Type I hexokinase, e.g., as a result of binding certain ligands [Smith and Wilson (1992) Arch. Biochem. Biophys. 292: 165-178]. rat; crossreactivity with Type I isozyme from other mammalian species has not been investigated. Monoclonal 1B2 recognizes an epitope in the C-terminal half of rat Type I hexokinase. Crossreactivity with the Type II or Type III isozymes is thought to be unlikely. Crossreactivity with the Type II or Type III isozymes is thought to be unlikely.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Type I isozyme of rat hexokinase
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Designation: Sp2/O-Ag14
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_528279 
Additional Information: The origin of the antibody is purified from rat brain. You can find details of the immunization and subsequent fusion protocol are found in Finney et al. (1984) J. Biol. Chem. 259: 8232-8237. Immunization of 100 micrograms of purified rat type I hexokinase was injected intraperitoneally and the adjuvant is freunds. RRID:AB_528279
Recommended Applications: ELISA, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1B2 JW was deposited to the DSHB by Wilson, J.E. (DSHB Hybridoma Product 1B2 JW)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

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