Clone ID / Name: 1B2 JW
Anti - hexokinase (Type I isozyme)
Catalog Fields
Identity
Clone ID / Name: 1B2 JW
Alternate Antibody Name: —
Antigen Name: hexokinase (Type I isozyme)
Alternate Antigen Name: —
Gene Symbol: —
Gene Name: —
Entrez Gene ID: —
Antigen Sequence: —
Antigen Species: rat
Antigen Mol. Weight: —
Uniprot ID: —
Human Protein Atlas: —
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG2b
Myeloma Strain: Designation: Sp2/O-Ag14
Antibody Registry / RRID: AB_528279
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: Type I isozyme of rat hexokinase
Immunogen Sequence: —
Epitope Mapped: No
Epitope Location / Seq: —
Clone: —
Additional Info: The origin of the antibody is purified from rat brain. You can find details of the immunization and subsequent fusion protocol are found in Finney et al. (1984) J. Biol. Chem. 259: 8232-8237. Immunization of 100 micrograms of purified rat type I hexokinase was injected intraperitoneally and the adjuvant is freunds. RRID:AB_528279
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Rat
Predicted Reactivity: —
Depositor
Depositor Name: J.E. Wilson
Institution: Michigan State University
Deposit Date: December 10, 2002
Depositor Notes: The antibody is reactive with the native enzyme (ELISA) but not the denatured form of the enzyme and thus is not useful for immunoblots. Binding of the antibody does not affect the kinetic properties of the rat Type I isozyme [Smith and Wilson (1992) Arch. Biochem. Biophys. 292: 165-178]. The epitope recognized is a ÒconformationalÓ (or ÒdiscontinuousÓ) epitope, and immunoreactivity is affected by conformational changes in the Type I hexokinase, e.g., as a result of binding certain ligands [Smith and Wilson (1992) Arch. Biochem. Biophys. 292: 165-178]. rat; crossreactivity with Type I isozyme from other mammalian species has not been investigated. Monoclonal 1B2 recognizes an epitope in the C-terminal half of rat Type I hexokinase. Crossreactivity with the Type II or Type III isozymes is thought to be unlikely. Crossreactivity with the Type II or Type III isozymes is thought to be unlikely.
Recommended Applications
ELISA
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
UniProt DataUniProtNo UniProt ID
▼No UniProt ID is available for this product.