1B5

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SKU: 1B5-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 1B5
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Itga2b/Itgb3
Ab Isotype: Armenian Hamster IgG
Gene Symbol:
Antibody Registry ID: AB_2617390 
Uniprot ID: Q9QUM0 O54890 
RRID:  
Entrez Gene ID: 16399 16416 
Clonality: Monoclonal
Immunogen: mouse platelets
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: No
Antigen Name: integrin alphaIIb/beta-3 (CD61) complex, mouse
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/2/2013
Antigen Molecular Weight: Predicted: 113 kDa (alphaIIb) and 87 kDa (beta3); Apparent: 140 kDa (alphaIIb) 90 kDa (beta3)
Depositor: Coller, B.S.
Antigen Sequence:
Depositor Institution: Rockefeller University, Laboratory of Blood and Vascular Biology
Antigen Species: mouse
Depositor Notes: This antibody doesn't appear to work for western blotting. Doesn't work on Human samples.
Host Species: Armenian hamster
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Mouse
Additional Information: 1B5 blocks the alphaIIbbeta3 receptor but not the alphaVbeta3 receptor.

In the original publication, the 1B5 isotype was determined to be Armenian hamster IgG3, kappa light chain.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, FACS, Function Blocking, Immunofluorescence, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1B5 was deposited to the DSHB by Coller, B.S. (DSHB Hybridoma Product 1B5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

9 References

  • Initial Publication
  • IF References
  • IP References
  • ELISA References
  • FB References
  • FACS References
  • All References
  • Initial Publication
    IF References
    IP References
    ELISA References
    FB References
    FACS References
    All References

    Dynamic platelet accumulation at the site of the occluded middle cerebral artery and in downstream microvessels is associated with loss of microvascular integrity after embolic middle cerebral artery occlusion.
    Chopp M
    Brain research 912.2 (2001 Sep 7): 181-94.

    Preparation of monoclonal antibodies to murine platelet glycoprotein IIb/IIIa (alphaIIbbeta3) and other proteins from hamster-mouse interspecies hybridomas.
    Coller BS
    Biochemical and biophysical research communications 262.1 (1999 Aug 19): 167-73.

    Structure and function of murine alphaIIbbeta3 (GPIIb/IIIa): studies using monoclonal antibodies and beta3-null mice,
    Coller BS
    Thrombosis and haemostasis 84.6 (2000 Dec): 1103-8.

    Dynamics of GPIIb/IIIa-mediated platelet-platelet interactions in platelet adhesion/thrombus formation on collagen in vitro as revealed by videomicroscopy.
    Coller BS
    Blood 101.3 (2003 Feb 1): 929-36.

    Megakaryocytes derived from embryonic stem cells implicate CalDAG-GEFI in integrin signaling.
    Shattil SJ
    Proceedings of the National Academy of Sciences of the United States of America 99.20 (2002 Oct 1): 12819-24.

    Variable protection of beta 3-integrin--deficient mice from thrombosis initiated by different mechanisms.
    Coller BS
    Blood 98.4 (2001 Aug 15): 1055-62.

    Leukocyte engagement of platelet glycoprotein Ibalpha via the integrin Mac-1 is critical for the biological response to vascular injury.
    Simon DI
    Circulation 112.19 (2005 Nov 8): 2993-3000.

    Relationships between Rap1b, affinity modulation of integrin alpha IIbbeta 3, and the actin cytoskeleton.
    Shattil SJ
    The Journal of biological chemistry 277.28 (2002 Jul 12): 25715-21.

    Morphological and functional platelet abnormalities in Berkeley sickle cell mice.
    Coller BS
    Blood cells, molecules & diseases 41.1 (2008 Jul-Aug): 109-18.

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