1B5

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$40.00
SKU: 1B5-s
View product citations for antibody 1B5 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: integrin alphaIIb/beta-3 (CD61) complex, mouse
Hybridoma Cells Available:
Antigen Species: mouse
Depositor: Coller, B.S.
Isotype: Armenian Hamster IgG
Antigen Sequence:
Host Species: Armenian hamster
Depositors Institution: Laboratory of Blood and Vascular Biology, Rockefeller University
Positive Tested Species Reactivity: Mouse
Depositors Notes: This antibody doesn't appear to work for western blotting. Doesn't work on Human samples.
Antigen Molecular Weight: Predicted: 113 kDa (alphaIIb) and 87 kDa (beta3); Apparent: 140 kDa (alphaIIb) 90 kDa (beta3)
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Itga2b/Itgb3
Immunogen: mouse platelets
Alternate Gene Names: CD41/CD61
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP2/0-Ag14
Epitope Location or Sequence:
Uniprot ID: Q9QUM0 O54890 
Immunogen Sequence: Full length protein
Entrez Gene ID: 16399 16416 
Additional Characterization:
Antibody Registry ID: AB_2617390 
Additional Information: 1B5 blocks the alphaIIbbeta3 receptor but not the alphaVbeta3 receptor.

In the original publication, the 1B5 isotype was determined to be Armenian hamster IgG3, kappa light chain.
Recommended Applications: ELISA, FACS, Function Blocking, Immunofluorescence, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1B5 was deposited to the DSHB by Coller, B.S. (DSHB Hybridoma Product 1B5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • IP References
  • ELISA References
  • FB References
  • FACS References
  • All References
  • Initial Publication
    IF References
    IP References
    ELISA References
    FB References
    FACS References
    All References

    Dynamic platelet accumulation at the site of the occluded middle cerebral artery and in downstream microvessels is associated with loss of microvascular integrity after embolic middle cerebral artery occlusion.
    Chopp M
    Brain research 912.2 (2001 Sep 7): 181-94.

    Preparation of monoclonal antibodies to murine platelet glycoprotein IIb/IIIa (alphaIIbbeta3) and other proteins from hamster-mouse interspecies hybridomas.
    Coller BS
    Biochemical and biophysical research communications 262.1 (1999 Aug 19): 167-73.

    Structure and function of murine alphaIIbbeta3 (GPIIb/IIIa): studies using monoclonal antibodies and beta3-null mice,.
    Coller BS
    Thrombosis and haemostasis 84.6 (2000 Dec): 1103-8.

    Dynamics of GPIIb/IIIa-mediated platelet-platelet interactions in platelet adhesion/thrombus formation on collagen in vitro as revealed by videomicroscopy.
    Coller BS
    Blood 101.3 (2003 Feb 1): 929-36.

    Megakaryocytes derived from embryonic stem cells implicate CalDAG-GEFI in integrin signaling.
    Shattil SJ
    Proceedings of the National Academy of Sciences of the United States of America 99.20 (2002 Oct 1): 12819-24.

    Variable protection of beta 3-integrin--deficient mice from thrombosis initiated by different mechanisms.
    Coller BS
    Blood 98.4 (2001 Aug 15): 1055-62.

    Leukocyte engagement of platelet glycoprotein Ibalpha via the integrin Mac-1 is critical for the biological response to vascular injury.
    Simon DI
    Circulation 112.19 (2005 Nov 8): 2993-3000.

    Relationships between Rap1b, affinity modulation of integrin alpha IIbbeta 3, and the actin cytoskeleton.
    Shattil SJ
    The Journal of biological chemistry 277.28 (2002 Jul 12): 25715-21.

    Morphological and functional platelet abnormalities in Berkeley sickle cell mice.
    Coller BS
    Blood cells, molecules and diseases 41.1 (2008 Jul-Aug): 109-18.

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