1B5
Catalog Fields
Antigen: integrin alphaIIb/beta-3 (CD61) complex, mouse
Hybridoma Cells Available: No
Antigen Species: mouse
Depositor: Coller, B.S.
Isotype: Armenian Hamster IgG
Antigen Sequence:
Host Species: Armenian hamster
Depositors Institution: Laboratory of Blood and Vascular Biology, Rockefeller University
Positive Tested Species Reactivity: Mouse
Depositors Notes: This antibody doesn't appear to work for western blotting. Doesn't work on Human samples.
Antigen Molecular Weight: Predicted: 113 kDa (alphaIIb) and 87 kDa (beta3); Apparent: 140 kDa (alphaIIb) 90 kDa (beta3)
Predicted Species Reactivity:
Gene: Itga2b/Itgb3
Immunogen: mouse platelets
Alternate Gene Names: CD41/CD61
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0-Ag14
Epitope Location or Sequence:
Uniprot ID: Q9QUM0 O54890
Immunogen Sequence: Full length protein
Entrez Gene ID: 16399 16416
Antibody Registry ID: AB_2617390
Additional Information: 1B5 blocks the alphaIIbbeta3 receptor but not the alphaVbeta3 receptor.
In the original publication, the 1B5 isotype was determined to be Armenian hamster IgG3, kappa light chain.
In the original publication, the 1B5 isotype was determined to be Armenian hamster IgG3, kappa light chain.
Recommended Applications: ELISA, FACS, Function Blocking, Immunofluorescence, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1B5 was deposited to the DSHB by Coller, B.S. (DSHB Hybridoma Product 1B5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
9 References
Initial Publication
Preparation of monoclonal antibodies to murine platelet glycoprotein IIb/IIIa (alphaIIbbeta3) and other proteins from hamster-mouse interspecies hybridomas.
Coller BS
Biochemical and biophysical research communications 262.1 (1999 Aug 19): 167-73.