Antigen: Secretory cells, cytoplasmic projections
Hybridoma Cells Available: Yes
Antigen Species: Schmidtea mediterranea
Depositor: Zayas, R.M.
Isotype: MIgM, kappa light chain
Depositors Institution: Department of Biology, San Diego State University
Host Species: mouse
Depositors Notes: Specific to secretory cell cytoplasmic projections
Positive Tested Species Reactivity: Schmidtea mediterranea
Immunogen: Formaldehyde-fixed cells from regenerating blastema of C1W4 clonal lineage
Myeloma Strain: F0
Antibody Registry ID: AB_2619606
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1B6-C4 was deposited to the DSHB by Zayas, R.M. (DSHB Hybridoma Product 1B6-C4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).