1B8-3D9

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$45.00 to $425.00
SKU: 1B8-3D9
View product citations for antibody 1B8-3D9 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Kettin (sallimus)
Available to For-Profits: Yes
Antigen Species: Honey Bee
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Saide, J.D.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila, Honeybee
Depositors Institution: Boston University
Antigen Molecular Weight: 2,065.8 kDa
Depositors Notes:
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Purified Z-discs
Gene: sls
Alternate Antibody Name:
Alternate Gene Names: titin, Dmel_CG1915
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NS-1
Epitope Mapped: No
Uniprot ID: Q9I7U4 
Epitope Location or Sequence:
Entrez Gene ID: 44013 
Immunogen Sequence:
Antibody Registry ID: AB_2721939 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Western Blot
Additional Information: FBgn0086906 RRID:AB_2721939
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1B8-3D9 was deposited to the DSHB by Saide, J.D. (DSHB Hybridoma Product 1B8-3D9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication
    IF References

    Characterization of components of Z-bands in the fibrillar flight muscle of Drosophila melanogaster.
    Pardue ML
    The Journal of cell biology 109.5 (1989 Nov): 2157-67.

    Z-band proteins in the flight muscle and leg muscle of the honeybee.
    Busquets-Turner L
    Journal of muscle research and cell motility 11.2 (1990 Apr): 125-36.

    WB References

    Characterization of components of Z-bands in the fibrillar flight muscle of Drosophila melanogaster.
    Pardue ML
    The Journal of cell biology 109.5 (1989 Nov): 2157-67.

    Z-band proteins in the flight muscle and leg muscle of the honeybee.
    Busquets-Turner L
    Journal of muscle research and cell motility 11.2 (1990 Apr): 125-36.

    All References

    Characterization of components of Z-bands in the fibrillar flight muscle of Drosophila melanogaster.
    Pardue ML
    The Journal of cell biology 109.5 (1989 Nov): 2157-67.

    Z-band proteins in the flight muscle and leg muscle of the honeybee.
    Busquets-Turner L
    Journal of muscle research and cell motility 11.2 (1990 Apr): 125-36.

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