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SKU: 1D3-3E4
View product citations for antibody 1D3-3E4 on CiteAb

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Available: 173

DSHB Data Sheet

Catalog Fields

Antigen: Zasp, PDZ and LIM domain protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Saide, J.D.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Boston University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody is also known as Z(210):1.
Antigen Molecular Weight: 238 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Zasp52
Immunogen: Purified Z-discs
Alternate Gene Names: Zasp, Dmel_CG30084,
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: A1ZA47 
Immunogen Sequence:
Entrez Gene ID: 36740 
Additional Characterization:
Antibody Registry ID: AB_2721940 
Additional Information: This antigen is also known as "Z band alternatively spliced PDZ-motif protein 52". FBgn0265991 RRID:AB_2721940
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1D3-3E4 was deposited to the DSHB by Saide, J.D. (DSHB Hybridoma Product 1D3-3E4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    All References

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