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SKU: 1D3-3E4
View product citations for antibody 1D3-3E4 on CiteAb

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Available: 154

DSHB Data Sheet

Catalog Fields

Antigen: Zasp, PDZ and LIM domain protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Saide, J.D.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Boston University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody is also known as Z(210):1.
Antigen Molecular Weight: 238 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Zasp52
Immunogen: Purified Z-discs
Alternate Gene Names: Zasp, Dmel_CG30084,
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: A1ZA47 
Immunogen Sequence:
Entrez Gene ID: 36740 
Additional Characterization:  
Antibody Registry ID: AB_2721940 
Additional Information: This antigen is also known as "Z band alternatively spliced PDZ-motif protein 52". FBgn0265991 RRID:AB_2721940
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1D3-3E4 was deposited to the DSHB by Saide, J.D. (DSHB Hybridoma Product 1D3-3E4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    All References

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