1D3-3E4

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SKU: 1D3-3E4

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 1D3-3E4
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Zasp52
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2721940 
Uniprot ID: A1ZA47 
RRID:  
Entrez Gene ID: 36740 
Clonality: Monoclonal
Immunogen: Purified Z-discs
Clone:
Immunogen Sequence:
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: Zasp, PDZ and LIM domain protein
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 10/28/2016
Antigen Molecular Weight: 238 kDa
Depositor: Saide, J.D.
Antigen Sequence:
Depositor Institution: Boston University
Antigen Species: Drosophila
Depositor Notes: This antibody is also known as Z(210):1.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: This antigen is also known as "Z band alternatively spliced PDZ-motif protein 52". FBgn0265991 RRID:AB_2721940
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1D3-3E4 was deposited to the DSHB by Saide, J.D. (DSHB Hybridoma Product 1D3-3E4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    All References

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