Antigen: Neuronal acetylcholine receptor subunit alpha-3
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Mihovilovic, Mirta
Host Species: mouse
Depositors Institution: Duke University
Positive Tested Species Reactivity: Human, Rodent
Depositors Notes: This antibody recognizes thymic cells.
Antigen Molecular Weight: 57.4 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Recombinant extracellular domain with boost of cytoplasmic domain for the alpha-3 AcchR subunit
Alternate Gene Names: LNCR2, NACHRA3, PAOD2
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P32297
Immunogen Sequence: aminio acids 1-127 of the alpha-3 AcChR subunit as extracellular domian and 313 to 411 as cytoplasmic domain of the alpha-3 subunit
Entrez Gene ID: 1136
Antibody Registry ID: AB_2721941
Additional Information: This antibody was first reported at the Annual Society for Neuroscience as an abstract/poster presentation. RRID:AB_2721941
Recommended Applications: ELISA, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1D4-9C was deposited to the DSHB by Mihovilovic, Mirta (DSHB Hybridoma Product 1D4-9C)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).