1D8 (reovirus)

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SKU: 1D8 (reovirus)
View product citations for antibody 1D8 (reovirus) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: reovirus sigma NS
Hybridoma Cells Available: Yes
Antigen Species: Mammalian orthoreovirus strain Type 3 Dearing (T3D)
Depositor: Dermody, T.S.
Isotype: MIgM, kappa light chain
Depositors Institution: University of Pittsburgh School of Medicine
Host Species: mouse
Depositors Notes: Immunoprecipitation, Immunostaining, western blot, flow cytometry, and functional inhibition are all not determined.
Positive Tested Species Reactivity: Type 3 Dearing (T3D)
Gene: M3
Antigen Molecular Weight: 41 kDa
Clonality: Monoclonal
Immunogen: Purified protein MBP-sigma NS-His
Immunogen Sequence: Full length sequence
Myeloma Strain: Sp2/0-Ag14
Additional Information: RRID:AB_2617401
Uniprot ID: Q9PY83 
Antibody Registry ID: AB_2617401 
Recommended Applications: ELISA
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1D8 (reovirus) was deposited to the DSHB by Dermody, T.S. (DSHB Hybridoma Product 1D8 (reovirus))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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