1G12

(0) No Reviews yet
$40.00
SKU: 1G12
View product citations for antibody 1G12 on CiteAb

In Stock

Available: 38

DSHB Data Sheet

Catalog Fields

Antigen: nidogen/entactin
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Halfter, W.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Department of Neurobiology, University of Pittsburgh
Positive Tested Species Reactivity: Avian, Chicken
Depositors Notes: Purification for the antibody is positive.
Antigen Molecular Weight: 160 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: chick retinal basal lamina
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: X63Ag8.653
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_2267323 
Additional Information: RRID:AB_2267323
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1G12 was deposited to the DSHB by Halfter, W.M. (DSHB Hybridoma Product 1G12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IHC References
  • All References
  • Initial Publication

    Composition, synthesis, and assembly of the embryonic chick retinal basal lamina.
    Cole GJ
    Developmental biology 220.2 (2000 Apr 15): 111-28.

    IHC References
    All References

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review