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1H9 Fibronectin Hep2 / 1H9B2

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$40.00
SKU: 1H9 Fibronectin Hep2 / 1H9B2
View product citations for antibody 1H9 Fibronectin Hep2 / 1H9B2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: fibronectin, canine, heparin binding region (Hep2)
Hybridoma Cells Available: Yes
Antigen Species: canine
Depositor: Burton-Wurster, N.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Baker Institute for Animal Health, College of Veterinary Medicine
Positive Tested Species Reactivity: Canine, Mouse
Depositors Notes: Specific for canine fibronectin.
Antigen Molecular Weight: Predicted (multiple isoforms):up to 265 kDa; Apparent: 205, 215, 225 and 230 kDa (multiple isoforms)
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: FN1
Immunogen: Purified canine cartilage fibronectin
Alternate Gene Names: FN
Alternate Antibody Name: 1H9A4, 1H9
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP 2/0
Epitope Location or Sequence: Type III repeat 14 of canine fibronectin (heparin binding region Hep2)
Uniprot ID: F1P6H7 
Immunogen Sequence: Full length protein
Entrez Gene ID: 403845 
Additional Characterization:
Antibody Registry ID: AB_2231928 
Additional Information: 1H9 was deemed to be canine-specific by the depositor because it doesn't appear to cross-react with human fibronectin. It has since been reported to work on mice fibronectin [PMID: 27621060].
Recommended Applications: ELISA, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1H9 Fibronectin Hep2 / 1H9B2 was deposited to the DSHB by Burton-Wurster, N. (DSHB Hybridoma Product 1H9 Fibronectin Hep2 / 1H9B2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    Molecular and immunologic differences in canine fibronectins from articular cartilage and plasma.
    Lust G
    Archives of biochemistry and biophysics 269.1 (1989 Feb 15): 32-45.

    IF References
    WB References

    Molecular and immunologic differences in canine fibronectins from articular cartilage and plasma.
    Lust G
    Archives of biochemistry and biophysics 269.1 (1989 Feb 15): 32-45.

    The cartilage-specific (V+C)- fibronectin isoform exists primarily in homodimeric and monomeric configurations.
    MacLeod JN
    The Biochemical journal 341 ( Pt 3). (1999 Aug 1): 555-61.

    ELISA References

    Molecular and immunologic differences in canine fibronectins from articular cartilage and plasma.
    Lust G
    Archives of biochemistry and biophysics 269.1 (1989 Feb 15): 32-45.

    Epitope Map References

    Molecular and immunologic differences in canine fibronectins from articular cartilage and plasma.
    Lust G
    Archives of biochemistry and biophysics 269.1 (1989 Feb 15): 32-45.

    All References

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