1H9 Fibronectin Hep2 / 1H9B2
Catalog Fields
Clone ID/Product Name: 1H9 Fibronectin Hep2 / 1H9B2
Available to For-Profits: Yes
Alternate Antibody Name: 1H9A4, 1H9
Gene Symbol: FN1
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2231928
Uniprot ID: F1P6H7
Entrez Gene ID: 403845
Clonality: Monoclonal
Immunogen: Purified canine cartilage fibronectin
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: SP 2/0
Epitope Mapped: Yes
Antigen Name: fibronectin, canine, heparin binding region (Hep2)
Epitope Location or Sequence: Type III repeat 14 of canine fibronectin (heparin binding region Hep2)
Alternate Antigen Name:
Deposit Date: 4/27/2005
Antigen Molecular Weight: Predicted (multiple isoforms):up to 265 kDa; Apparent: 205, 215, 225 and 230 kDa (multiple isoforms)
Depositor: Burton-Wurster, N.
Antigen Sequence:
Depositor Institution: Cornell University, Baker Institute for Animal Health, College of Veterinary Medicine
Antigen Species: canine
Depositor Notes: Specific for canine fibronectin.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Canine, Mouse
Additional Information: 1H9 was deemed to be canine-specific by the depositor because it doesn't appear to cross-react with human fibronectin. It has since been reported to work on mice fibronectin [PMID: 27621060].
Recommended Applications: ELISA, Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1H9 Fibronectin Hep2 / 1H9B2 was deposited to the DSHB by Burton-Wurster, N. (DSHB Hybridoma Product 1H9 Fibronectin Hep2 / 1H9B2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
3 References
Initial Publication
Molecular and immunologic differences in canine fibronectins from articular cartilage and plasma.
Lust G
Archives of biochemistry and biophysics 269.1 (1989 Feb 15): 32-45.